Abstract: A fluorescence polarization process used to identify activity of conjugative enzymes involved in xenobiotic transformations, such as glucuronosyltransferases is provided.
Abstract: The system comprises mixing a fluorescence-emitting compound that binds to the steroid hormone receptors in a solution containing the steroid hormone receptors. Then, measuring the fluorescence polarization of the solution. Subsequently, incubating the solution with at least one molecule that may compete with the compound for interaction with the steroid hormone receptors. Measuring the fluorescence polarization of the solution again. Finally, comparing the fluorescence polarization measurements to quantify any competitive interaction.
Type:
Grant
Filed:
December 20, 1999
Date of Patent:
January 28, 2003
Assignee:
PanVera LLC
Inventors:
Thomas J. Burke, Randall E. Bolger, Kerry M. Ervin, Robert G. Lowery, William J. Checovich
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