Abstract: The invention relates to a method for determining the florescence of antibodies on exosomes using quality control of the measurement using two different control methods.
Abstract: The invention relates to a device and method for reducing the reduction in intensity of a fluorescence dye by laser light when determining fluorescence and the number of antibodies on exosomes, comprising means for storing different measurement points of various differently coloured lasers in a measuring cell at certain measurement positions, the focusing of the laser beam interacting with the sample being recorded in a video camera as the centre of a convergent beam bundle.
Type:
Application
Filed:
July 6, 2020
Publication date:
July 28, 2022
Applicant:
PARTICLE METRIX GMBH
Inventors:
Hanno WACHERNIG, Clemens HELMBRECHT, Jens SCHIFFMANN
Abstract: The invention relates to a device and method for measuring the concentration, size and zeta potential of nanoparticles in liquids in scattered light mode and fluorescence mode, comprising the following features: a) a sample (28) is irradiated from above by a laser (1) via a beamsplitter (14) and an adjustable-focus liquid lens (9), and the reflected beam of light is deflected by the same beamsplitter (14) and a further liquid lens (8) onto a detector (5), and analysed; b) to observe the fluorescent light, a fluorescence filter (19) is connected in the convergent beam path between the liquid lens (8) and the detector (5), to increase the distance between the scattered light plane (31) and the fluorescence plane (30); and c) to control the measurement process, a particle tracking program, an optical control unit (15) and a display (2) with a touch screen are used.
Abstract: The invention relates to a device and method for measuring the concentration, size and zeta potential of nanoparticles in liquids in scattered light mode and fluorescence mode, comprising the following features: a) a sample (28) is irradiated from above by a laser (1) via a beamsplitter (14) and an adjustable-focus liquid lens (9), and the reflected beam of light is deflected by the same beamsplitter (14) and a further liquid lens (8) onto a detector (5), and analysed; b) to observe the fluorescent light, a fluorescence filter (19) is connected in the convergent beam path between the liquid lens (8) and the detector (5), to increase the distance between the scattered light plane (31) and the fluorescence plane (30); and c) to control the measurement process, a particle tracking program, an optical control unit (15) and a display (2) with a touch screen are used.
Abstract: A method and device for optically detecting particles, including: (a) a cell wall of rectangular cross-section is fitted on a longitudinal surface and adjoining transverse surface with an L-shaped heating and cooling element; (b) the center of the transverse surface of the cell wall opposite the transverse surface which forms the support of the cell wall is irradiated by an irradiation device and is observed at right angles to the optical axis of the irradiation device; (c) the focus of the irradiation device and the observation device can be moved by a motor to any point in the three-dimensional inner region defined by the cell wall; (d) the surface of the cell wall opposite the optical glass window through which the radiation from the irradiation device enters comprises another optical glass window; (e) the temperature of the surface of the cell wall is monitored by two thermistors.
Abstract: A method and device for optically detecting particles (23) have the following features: (a) a cell wall (9) of rectangular cross-section, made of black glass, is fitted on a longitudinal surface and adjoining transverse surface with an L-shaped heating and cooling element (1); (b) the centre of the transverse surface of the cell wall (9) opposite the transverse surface which forms the support of the cell wall (9) is irradiated by an irradiation device and is observed at right angles to the optical axis of the irradiation device by means of an observation device; (c) the focus of the irradiation device and the focus of the observation device can be moved by a motor to any point in the three-dimensional inner region defined by the cell wall (9) by means of a control device; (d) the surface of the cell wall (9) opposite the optical glass window (11) through which the radiation from the irradiation device enters comprises another optical glass window (11) in the centre thereof; (e) the temperature of the surface