Abstract: This invention is on manufacturing method of antimicrobial and anti-fungus wet tissues with antimicrobial and anti-fungus whole cloth for wet tissue(i.e. non-woven fabrics, cottons and papers) manufactured with nano metal particles soak in tissue manufacturing water with nano sized metal particles which have antimicrobial and anti-fungus function to have multiple effects. According to this invention, woven fabrics, cottons and papers soak in the tissue manufacturing water that one or more than one of selected nano metal particles from gold, platinum, silver, germanium, selenium, zinc, copper and tungsten are mixed in the tissue manufacturing water and then they have antimicrobial and anti-fungus function.