Abstract: This invention provides a reaction mixture to amplify a nucleic acid. The mixture includes a double stranded DNA molecule with one or more primer recognition sites and a mutant RNA polymerase designed to transcribe deoxynucleotides.
Abstract: This invention provides for a novel amplification procedure for nucleic acid. The method uses a wild type or mutant RNA polymerase designed to transcribe both deoxyribonucleotides and ribonucleotides.