Abstract: A dye produced from tobacco plant materials selected from tobacco plants are described and a process for making the dye from the tobacco plants. In a preferred embodiment, the tobacco plants are organically grown and the stems and leaves of the plants are utilized to produce the dye. In one embodiment, the process percolates a hot liquid solution onto the plant materials to produce tobacco plant fiber and the dye. In the preferred embodiment, the process includes the steps of grinding the plant materials, adding treated water to form a solution, adding plant enzymes, heating the solution for set periods of time and selectively adjusting the pH level of the solution. The dye can be produced in many colors and does not require a mordant to bind. The process does not produce waste. The process expands the use of tobacco plants, minimizes environmental impacts and requires less material, fuel and other resources.