Abstract: A method is provided for genotyping a target sequence at at least two allelic sites by a 5' nuclease amplification reaction. In one embodiment, the method includes performing a nucleic acid amplification on a target sequence having at least two different allelic sites using a nucleic acid polymerase having 5'.fwdarw.

Abstract: A system and method for identifying lanes in images generated from DNA sequencing and fragment analysis is described. One example method includes a computer implemented method of determining the locations of lanes of separated samples. Each lane corresponds to a sample being separated by flowing the sample through a media. The method includes the following elements. Place some samples on the media. Cause the samples to separate into the lanes. Create a digital image of the lanes. Fit some curves to the lane images. Each curve corresponds to a lane formed from a separated sample.