Abstract: A frame holder for a stereotactic frame holds the stereotactic frame in proper alignment with the head coil of an imaging unit so the reference markers on the stereotactic frame are properly aligned with the axis of the head coil. The frame holder has a base member that receives the base ring of the stereotactic frame so the frame holder and the stereotactic frame are locked together with proper alignment of the reference markers. A top member is spaced from the base member, and has a support block to carry the weight of the stereotactic frame. Posts fix the top member to the base member. The base and top member are sized and shaped to be snugly, slidably received within the opening in the head coil.