Abstract: Embodiments of the present invention describe primer pairs, methods and kits for identifying and/or detecting killer-cell immunoglobulin-like receptor (KIR) alleles. The present primer sets include one or more primer pairs that can produce amplicons specific for an individual KIR allele and that are less than 1000 bp in size. Additionally, the primer sets can target intra-exon and/or extracellular domains of KIR alleles for amplification.
Abstract: The present invention provides methods for analyzing polymorphic nucleic acids using duplex separation and/or identification techniques. In the present methods one of the nucleic acids has a sequence which includes universal bases that correspond in position to one or more of the polymorphic positions of the polymorphic nucleic acid. The nucleic acid including the universal bases can either represent the polymorphic nucleic acid and hybridize with a reference strand, or act as a reference strand and hybridize with the polymorphic nucleic acid directly, to form a duplex. Separation and/or identification of the duplex from other duplexes and materials can then be performed.
Abstract: An antibody raised against an epitope of a peptide having an amino acid sequence selected from a group consisting of Leu Asp Gly Tyr Pro Ser Asp Tyr Asp Pro Phe Val Glu Asn Ala Gln Ile Tyr (SEQ ID NO:1) and Leu Asp Gly Tyr Pro Ser Asp Tyr Asp Pro Phe Glu Glu Asn Ala Gln Ile Tyr (SEQ ID NO:2) is advantageously used in an indirect immunoassay to detect the presence of active Human Herpesvirus-6 in a patient sample.
Type:
Grant
Filed:
May 26, 1998
Date of Patent:
August 15, 2000
Assignee:
Pel-Freez Rabbit Meat, Inc.
Inventors:
Gary L. Milburn, Robert E. Novy, Jr., Robert C. Mierendorf, Jr.
Abstract: Recombinant rabbit tissue factor is cloned and produced in a bacterial host. This protein, which is relatively insoluble and has several disulfide bonds, requires special modifications in order to express and be purified at commercial levels. By expressing the tissue factor as a fusion protein with a bacterial enzyme, thioredoxin, solubility is increased. Use of a thioredoxin reductase deficient host aids in proper tertiary structure for biological activity.
Type:
Grant
Filed:
July 16, 1996
Date of Patent:
January 12, 1999
Assignee:
Pel-Freez
Inventors:
Robert E. Novy, Jr., Michael J. Domanico, Keith W. Yaeger, Warren Kroeker
Abstract: An antibody raised against an epitope of a peptide having an amino acid sequence selected from a group consisting of LDGYPSDYDPFVENAQIY (SEQ ID NO:1) and LDGYPSDYDPFEENAQIY (SEQ ID NO:2) is advantageously used in an indirect immunoassay to detect the presence of active Human Herpesvirus-6 in a patient sample.
Type:
Grant
Filed:
July 16, 1996
Date of Patent:
May 26, 1998
Assignee:
Pel-Freez Rabbit Meat, Inc.
Inventors:
Gary L. Milburn, Robert E. Novy, Jr., Robert C. Mierendorf, Jr.