Abstract: A bioreactor system includes a bioreaction unit configured to accommodate a culture medium, and a treatment unit configured to recycle the culture medium transferred from the bioreaction unit, wherein the treatment unit includes a treatment tank which accommodates the culture medium transferred from the bioreaction unit, a culture medium exchange part configured to exchange the culture medium between the bioreaction unit and the treatment tank, and a waste product removal part configured to remove waste products contained in the culture medium accommodated in the treatment tank.

Abstract: The disclosure relates to a composition for detecting a biomarker to predict differentiation potential of a muscle stem cell, including an agent for measuring an mRNA expression level or protein activity level of CD56. Particularly, it is confirmed that among muscle stem cells, cells with high expression or activity levels of CD56 have excellent differentiation potential into myotubes even after continued subculture, and the differentiation potential of muscle stem cells according to the culture period can be predicted in advance, so that it can be usefully used as a tool for effectively evaluating and managing the quality of muscle stem cells.

Abstract: A method for aligning and culturing cells in three dimensions includes mixing cells with a hydrogel to prepare a bio-ink, outputting the bio-ink while moving a nozzle of a discharge device to form an output product having a pattern having at least parallel portions, curing the output product, and culturing the cured product. According to the present invention, cells may be cultured to a high density in a three-dimensionally aligned state along a parallel pattern, so that there is an advantage in that the texture of a tissue formed by the cells, for example, a unique texture of a muscle tissue, may be simulated or reproduced.

Abstract: Disclosed is a bioreactor including a rotation device that rotates a reaction container while a reference direction is taken as an axial direction thereof, and an angle adjusting device coupled to the rotation device to adjust an angle defined by the reference direction, which is dependent on an arrangement posture of the rotation device, with respect to a ground surface, by changing the arrangement posture of the rotation device.

Abstract: The present invention relates to a method of manufacturing a spheroid using a composition for a liquid bath for dipping a hydrogel droplet and to a spheroid manufactured thereby. The method of manufacturing the spheroid according to the present invention is capable of successfully forming a spheroid having a 3D spherical shape that is difficult to implement on a planar substrate and also of realizing a spheroid having a uniform size, and thus can be effectively used as a technique for obtaining a spheroid having a 3D spherical shape and a uniform size in the field of cell culture.

Abstract: The present invention relates to a method of manufacturing a spheroid using a composition for a liquid bath for dipping a hydrogel droplet and to a spheroid manufactured thereby. The method of manufacturing the spheroid according to the present invention is capable of successfully forming a spheroid having a 3D spherical shape that is difficult to implement on a planar substrate and also of realizing a spheroid having a uniform size, and thus can be effectively used as a technique for obtaining a spheroid having a 3D spherical shape and a uniform size in the field of cell culture.