Abstract: Novel peptide nucleic acids and novel linked peptide nucleic acids, form triple stranded structures with nucleic acids. The peptide nucleic acids include ligands such as naturally occurring nucleobases attached to the peptide backbone through a suitable linker. Other nucleobases including C-pyrimidines and iso-pyrimidines can be used as the ligands in Hoogsteen strands to increase binding affinity. Two peptide nucleic acid strands are joined together with a linker to form a bis-peptide nucleic acid. The individual strands of the peptide nucleic acids in the bis compounds can be oriented either parallel or antiparallel to each other.
June 28, 1998
Date of Patent:
August 27, 2002
ISIS Pharmaceuticals, Inc., PepSeptive Biosystems, Inc.
Michael Egholm, Peter Nielsen, Ole Buchardt, Kim L. Dueholm, Leif Christensen, James M. Coull, John Kiely, Michael Griffith