Abstract: The present disclosure relates to a system and a method for automatically measuring and assessing hemodynamics in tissue of an anatomical structure of a subject. In particular the present disclosure relates to continuously measuring and assessing hemodynamics in medical procedures using fluorescence imaging and wherein the administration of the fluorescent agent is controlled and automated. One aspect relates to a method of automatic perfusion assessment of an anatomical structure of a subject, the method comprising administration into a vein of a bolus corresponding to less than 0.005 mg ICG/kg body weight of a first fluorescence imaging agent.

Abstract: A system and a method for automatically and continuously measuring and assessing hemodynamics uses fluorescence imaging in which the fluorescent agent is controlled and automated. A method of automatic perfusion assessment of an anatomical structure of a subject includes administration into a vein of a bolus corresponding to less than 0.01 mg ICG/kg body weight of a first fluorescence imaging agent. A system for automatic perfusion assessment during a medical procedure includes a controllable injection pump for holding at least one first fluorescence imaging agent, the injection pump being configured for injecting a predefined amount of the first fluorescence imaging agent into the blood. The system is configured for receiving and analysing a time series of fluorescence images and determining at least one perfusion parameter based on the analysis.