Patents Assigned to Perkin-Elmer
  • Acetic anhydride activation for C-terminal protein sequencing
    Patent number: 5468843
    Abstract: A method is provided for C-terminal sequencing of a protein or peptide. An important feature of the method is the formation of an oxazolone moiety at the C-terminus of a protein or peptide by treatment with acetic anhydride under basic conditions followed by conversion of the oxazolone to a thiohydantoin moiety by treatment with thiocyanate under acidic conditions. Yields of thiohydantoin are further enhanced by delivering thiocyanate as the conjugate acid of a sterically hindered alkylammnonium cation.
    Type: Grant
    Filed: September 8, 1994
    Date of Patent: November 21, 1995
    Assignee: Perkin-Elmer
    Inventors: Victoria L. Boyd, MeriLisa Bozzini, G. Marc Loudon