Abstract: The invention relates to a cellobiose phosphorylase, which catalyzes, among other things, the synthesis of cellobiose from glucose 1-phosphate and glucose. The cellobiose phosphorylase according to the invention can be understood as a mutation of the cellobiose phosphorylase from Cellulomonas uda. In comparison to cellobiose phosphorylase of the wild type, the cellobiose phosphorylase according to the invention is distinguished by improved activity and process stability, in particular temperature stability, and lower product inhibition and therefore is especially suitable for use in industrial processes.
Type:
Grant
Filed:
September 10, 2015
Date of Patent:
December 3, 2019
Assignee:
PFEIFER & LANGEN GMBH & CO. KG
Inventors:
Timo Johannes Koch, Thomas Hässler, Birgit Brucher, Andreas Vogel
Abstract: The invention relates to a process for the synthesis of a product saccharide, preferably of D-allulose from an educt saccharide, preferably from D-fructose under heterogeneous or homogeneous catalysis which includes chemical and/or enzymatic catalysis. The synthesis is performed in at least two reactors that are arranged in series and the reaction product exiting the first reactor is subjected to chromatographic separation before it enters the second reactor. Preferably, the chromatographic separation is integrated in a simulated moving bed.
Type:
Application
Filed:
November 9, 2017
Publication date:
October 17, 2019
Applicant:
PFEIFER & LANGEN GMBH & Co. KG
Inventors:
Timo Johannes Koch, Florian Kipping, Steffen Butz, Marcel Lesch, Thomas Hässler