Patents Assigned to Pfenex Inc.
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Publication number: 20140162279Abstract: This invention is a process for improving the production levels of recombinant proteins or peptides or improving the level of active recombinant proteins or peptides expressed in host cells. The invention is a process of comparing two genetic profiles of a cell that expresses a recombinant protein and modifying the cell to change the expression of a gene product that is upregulated in response to the recombinant protein expression. The process can improve protein production or can improve protein quality, for example, by increasing solubility of a recombinant protein.Type: ApplicationFiled: November 4, 2013Publication date: June 12, 2014Applicant: PFENEX INC.Inventors: THOMAS M. RAMSEIER, HONGFAN JIN, CHARLES H. SQUIRES
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Publication number: 20140051093Abstract: The present invention relates to the field of recombinant toxin protein production in bacterial hosts. In particular, the present invention relates to production processes for obtaining high levels of a recombinant CRM197, Diphtheria Toxin, Pertussis Toxin, Tetanus Toxoid Fragment C, Cholera Toxin B, Cholera holotoxin, and Pseudomonas Exotoxin A, from a bacterial host.Type: ApplicationFiled: July 26, 2013Publication date: February 20, 2014Applicant: Pfenex Inc.Inventors: Diane M. Retallack, Lawrence Chew
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Publication number: 20140051841Abstract: The present invention relates to processes for purifying high-quality recombinant Plasmodium falciparum circumsporozoite protein at high yields.Type: ApplicationFiled: March 15, 2013Publication date: February 20, 2014Applicant: PFENEX INC.Inventors: Jeffrey Allen, Greg Cantin, Ryan Haverstock
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Patent number: 8603824Abstract: This invention is a process for improving the production levels of recombinant proteins or peptides or improving the level of active recombinant proteins or peptides expressed in host cells. The invention is a process of comparing two genetic profiles of a cell that expresses a recombinant protein and modifying the cell to change the expression of a gene product that is upregulated in response to the recombinant protein expression. The process can improve protein production or can improve protein quality, for example, by increasing solubility of a recombinant protein.Type: GrantFiled: July 26, 2005Date of Patent: December 10, 2013Assignee: Pfenex, Inc.Inventors: Thomas M. Ramseier, Hongfan Jin, Charles H. Squires
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Patent number: 8569015Abstract: An optimized synthetic polynucleotide encoding a Bacillus anthracis protective antigen and an anthrax vaccine based on the encoded protective antigen. Furthermore, heterologous expression in a host Pseudomonas fluorescens bacteria of an optimized polynucleotide sequence encoding a Bacillus anthracis protective antigen.Type: GrantFiled: May 30, 2007Date of Patent: October 29, 2013Assignee: Pfenex Inc.Inventors: Lada L. Rasochova, Philip P. Dao, Jamie P. Phelps
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Patent number: 8530171Abstract: The present invention relates to the field of recombinant toxin protein production in bacterial hosts. In particular, the present invention relates to production processes for obtaining high levels of a recombinant CRM197, Diphtheria Toxin, Pertussis Toxin, Tetanus Toxoid Fragment C, Cholera Toxin B, Cholera holotoxin, and Pseudomonas Exotoxin A, from a bacterial host.Type: GrantFiled: March 28, 2011Date of Patent: September 10, 2013Assignee: Pfenex Inc.Inventors: Diane M. Retallack, Lawrence Chew, Hongfan Jin, Henry W. Talbot
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Patent number: 8455218Abstract: The present invention relates to the field of recombinant protein production in bacterial hosts. It further relates to expression of soluble, active recombinant protein by using secretion signals to direct the protein to the periplasmic space of a bacterial cell. In particular, the present invention relates to a production process for obtaining soluble hG-CSF protein from a bacterial host.Type: GrantFiled: March 30, 2011Date of Patent: June 4, 2013Assignee: Pfenex, Inc.Inventors: Hongfan Jin, Lawrence Chew
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Patent number: 8318481Abstract: Provided herein are improved copy number plasmids, particularly those plasmids capable of replication in a bacterial cell. The improved copy number plasmid contain a deletion, insertion, or substitution in the replication control region, particularly a Pseudomonas-specific replication control region, that results in an increase in plasmid copy number in comparison to a control plasmid. Also provided are host cells containing the improved copy number plasmids, as well as methods of using the improved copy number plasmids for the recombinant production of a protein of interest. Further provided are methods for generating plasmids with improved copy number. The methods disclosed herein involve the reiterative selection of improved copy number plasmids by the growth and selection of plasmids capable of growth under increasing selective pressure, wherein the selective pressure is applied utilizing a selection agent to which the control plasmid confers resistance.Type: GrantFiled: December 5, 2008Date of Patent: November 27, 2012Assignee: Pfenex Inc.Inventors: Lawrence C. Chew, Stacey L. Lee
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Patent number: 8288127Abstract: The present invention provides an improved expression system for the production of recombinant polypeptides utilizing auxotrophic selectable markers. In addition, the present invention provides improved recombinant protein production in host cells through the improved regulation of expression.Type: GrantFiled: July 30, 2009Date of Patent: October 16, 2012Assignee: Pfenex, IncInventors: Jane C. Schneider, Lawrence C. Chew, Anne Kathryn Badgley, Thomas Martin Ramseier
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Publication number: 20110287443Abstract: The present invention relates to the field of recombinant toxin protein production in bacterial hosts. In particular, the present invention relates to production processes for obtaining high levels of a recombinant CRM197, Diphtheria Toxin, Pertussis Toxin, Tetanus Toxoid Fragment C, Cholera Toxin B, Cholera holotoxin, and Pseudomonas Exotoxin A, from a bacterial host.Type: ApplicationFiled: March 28, 2011Publication date: November 24, 2011Applicant: Pfenex Inc.Inventors: Diane M. Retallack, Lawrence Chew, Hongfan Jin, Henry W. Talbot
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Publication number: 20110245474Abstract: The present invention relates to the field of recombinant protein production in bacterial hosts. It further relates to expression of soluble, active recombinant protein by using secretion signals to direct the protein to the periplasmic space of a bacterial cell. In particular, the present invention relates to a production process for obtaining soluble hG-CSF protein from a bacterial host.Type: ApplicationFiled: March 30, 2011Publication date: October 6, 2011Applicant: Pfenex Inc.Inventors: Hongfan Jin, Lawrence Chew
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Patent number: 8017355Abstract: The present invention provides methods for producing recombinant peptides in a bacterial host utilizing a mannitol, arabitol, glucitol, or glycerol-inducible promoter, wherein the host bacterial cell that produces the peptide has been rendered incapable of degrading or metabolizing mannitol, arabitol, or glucitol, or derivatives or analogues thereof. The present invention provides bacterial cells that have been genetically altered to inhibit the metabolism or degradation of mannitol, glucitol, or arabitol, or derivatives or analogues thereof. The present invention utilizes mannitol, arabitol, glucitol, or glycerol to induce expression of a target polypeptide from an inducible promoter, allowing for the use of an inexpensive and stable carbon source inducer in the fermentation processes for the production of recombinant peptides.Type: GrantFiled: December 9, 2008Date of Patent: September 13, 2011Assignee: Pfenex, Inc.Inventors: J. Carrie Schneider, Bettina Rosner
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Publication number: 20110217784Abstract: The present invention relates to the field of recombinant protein production in bacterial hosts. It further relates to extraction of soluble, active recombinant protein from an insoluble fraction without the use of denaturation and without the need for a refolding step. In particular, the present invention relates to a production process for obtaining high levels a soluble recombinant Type 1 interferon protein from a bacterial host.Type: ApplicationFiled: March 2, 2011Publication date: September 8, 2011Applicant: Pfenex, Inc.Inventors: Jeffrey Allen, Ping-Hua Feng, Anant Patkar, Keith L. Haney, Lawrence Chew, Lei Lei Phokham Sengchanthalangsy
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Patent number: 7985564Abstract: Improved methods and prokaryotic expression systems for producing recombinant proteins using Sec-system secretion signal peptides are described using Pseudomonas fluorescens Sec secretion systems. Specific novel Sec-system secretion signal peptides are described, as are fusion proteins and coding sequences for improved secretion of recombinant proteins and peptides.Type: GrantFiled: November 22, 2004Date of Patent: July 26, 2011Assignee: Pfenex, Inc.Inventors: Diane M. Retallack, Jane C. Schneider, Thomas Martin Ramseier
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Publication number: 20110111977Abstract: Methods of identifying and expressing an antibody variant are disclosed wherein the method comprises identifying a binding region in an antibody, fusing the binding region to a plurality of scaffolds of antibody constant regions to obtain antibody fragment variants, expressing the antibody fragment variants in organisms to form constructs and expressing the constructs carried by the organisms to form induced cultures, wherein the organisms are expressed in HTP mode.Type: ApplicationFiled: July 1, 2009Publication date: May 12, 2011Applicant: Pfenex, Inc.Inventor: Diane Retallack
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Publication number: 20110020868Abstract: Compositions and methods for improving expression and/or secretion of a polypeptide of interest in a host cell are provided. Compositions including a coding sequence for a bacterial secretion signal peptide are provided. The compositions of the invention are useful for increasing accumulation of properly processed proteins in the periplasmic space of a host cell, or for increasing secretion of properly processed proteins. In particular, isolated secretion signal peptide-encoding nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the nucleic acid molecules are encompassed. The present invention provides for isolated nucleic acid molecules including nucleotide sequences encoding the amino acid sequences shown in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, and 24, and the nucleotide sequences set forth in SEQ ID NO: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, and 23, as well as variants and fragments thereof.Type: ApplicationFiled: September 16, 2010Publication date: January 27, 2011Applicant: PFENEX, INC.Inventors: Russell J. Coleman, Diane Retallack, Stacey L. Lee, Charles D. Hershberger
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Patent number: 7833752Abstract: Compositions and methods for improving expression and/or secretion of a polypeptide of interest in a host cell are provided. Compositions including a coding sequence for a bacterial secretion signal peptide are provided. The compositions of the invention are useful for increasing accumulation of properly processed proteins in the periplasmic space of a host cell, or for increasing secretion of properly processed proteins. In particular, isolated secretion signal peptide-encoding nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the nucleic acid molecules are encompassed. The present invention provides for isolated nucleic acid molecules including nucleotide sequences encoding the amino acid sequences shown in SEQ ID NO:2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, and 24, and the nucleotide sequences set forth in SEQ ID NO:1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, and 23, as well as variants and fragments thereof.Type: GrantFiled: October 22, 2009Date of Patent: November 16, 2010Assignee: Pfenex, Inc.Inventors: Russell J. Coleman, Diane M. Retallack, Stacey L. Lee, Charles D. Hershberger
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Patent number: 7794972Abstract: Novel benzoate- or anthranilate-inducible promoters, and novel tandem promoters, and variants and improved mutants thereof, useful for commercial prokaryotic fermentation systems, nucleic acid constructs containing the promoters, expression systems using them, methods for expressing proteins by use thereof, and proteins expressed thereby.Type: GrantFiled: January 3, 2005Date of Patent: September 14, 2010Assignee: Pfenex Inc.Inventors: Diane M. Retallack, Venkiteswaran Subramanian