Abstract: A method of analyzing a polynucleotide of interest, comprising providing one or more sets of consecutive oligonucleotide primers differing within each set by one base at the growing end therof; annealing a single strand of the polynucleotide or a fragment of the polynucleotide to the oligonucleotide primers under hybridization conditions; subjecting the primers to single base extension reactions with a polymerase and terminating nucleotides, the terminating nucleotides being mutually distinguishable; and observing the location and identity of each terminating nucleotide to thereby analyze the sequence or a part of the nucleotide sequence of the polynucleotide of interest, is disclosed. An apparatus comprising a solid support to which is attached at defined locations thereon one or more sets of consecutive oligonucleotide primers differing within each set by one base at the growing end thereof is also described.

Abstract: Nucleoside analogues have structure (2) wherein Q is H or a sugar moiety or sugar analogue or a modified sugar or a nucleic backbone or backbone analogue, W is an alkylene or alkenylene chain of 0-5 carbon atoms, any of which may carry a substituent R8, X is O or N or NR12 or CR10, X′ is O or S or N, provided that when X′ is O or S, then X is C, Y is CH or N, R6 is NH2 or SMe or SO2Me or NHNH2, each of R7 and R8 is independently H or F or alkyl or alkenyl or aryl or acyl or a reporter moiety, R12 is independently H or alkyl or alkenyl or aryl or acyl or a reporter moiety, and R10 is H or ═O or F or alkyl or aryl or a reporter moiety.

Abstract: A cassette and electrophoretic gel assembly includes a non-conductive cassette, two solid buffer reservoirs, and an agarose gel. The assembly is disposable, and the sample wells on the gel are in standard microtiter plate format. The configuration is such that the gel is continuously in electrical contact with the electrodes in operation despite the production/migration of water and other exudates.

Abstract: An access valve suitable for controlling fluid flow into and out of a chromatography column has a central axially movable probe with a head acting as a spool valve in a barrel. Axial movement of the probe adjusts the valve between a fully open condition, in which bota first conduit extending through the probe and a second conduit defined around the probe are open to the column interior, a partly open condition in which a second sealing land on the probe closes the second conduit, and a fully closed position in which both conduits are closed. The three positions are useful for packing and unpacking chromatography media into and from the column. In the closed condition of the valve, the first and second conduits communicate with one another so that the valve interior can be cleaned while the column is operating.

Abstract: An improved system for running electrophoresis gels “face up” is described. The system includes a single gel capacity strip holder, two electrodes, a sample cup, and a cover. The system provides a means of separating basic proteins on any length gel strip and the cup provides a user-friendly means of successfully putting the proteins on the gel face.

Abstract: A sliding valve having a flow line on which there may be placed a module having flow channel that can be connected and disconnected. The valve avoids the problem of liquid remaining in the flow channel of a first valve component by providing flow channel intersecting segment which intersects both components of the sliding valve so that in a module disconnecting orientation, each valve component defines separate segments of the flow channel while in the connected orientation, the valve components define a single flow channel. The construction allows for liquid flow through the valve independent of the module being in connected or disconnected position.

Abstract: A purified recombinant thermostable DNA polymerase polymerase which exhibits at least about 80% activity at salt concentations of 50 mM and greater, at least about 70% activity at salt concentrations of 25 mM and greater, and having a processivity of about 30 nucleotides per binding event. An isolated nucleic acid that encodes the thermostable DNA polymerase, as well as a recombinant DNA vector comprising the nucleic acid and a recombinant host cell transformed with the vector, are also disclosed. A method of sequencing DNA using the DNA polymerase as well as a kit for sequencing DNA is also disclosed.

Abstract: A method for the separation of a predetermined compound comprising i) partitioning the compound in a two-phase (system A) in which at least one of the phases is rich (phase 1) and the other is poor (phase 2) in a thermoseparating polymer (I), ii) collecting one phase (phase 1 or phase 2) containing said compound, and iii if desired, further working up said compound from the phase collected in step ii, characterized a) in that polymer (I) is a micell-forming thermoseparating polymer, and b) in that phase 2 possibly contains at least one agent that is cloud point-decreasing for thermoseparating polymer (I), such as a polymer (II) that is incompatible with polymer (I) or a salt.

Abstract: A fraction collector (1) for dispensing liquid from a liquid source into receptacles (23) carried on a rotatable turntable (6), wherein the turntable is connected to the fraction collector casing (5) via a support arm (7) such that the turntable is rotatably mounted at one end of said support arm, and said support arm at its other end being rotatably mounted to the fraction collector casing. Thereby it is possible to align any position of the turntable under a dispensing tube (4) to dispense a liquid into a receptacle placed anywhere on the turntable.

Abstract: A method for detecting a mutation in a target nucleic acid sequence that comprises attaching oligonucleotide primers to a substrate, wherein the oligonucleotide primers have a sequence that is complementary to the target nucleic acid sequence, and wherein the oligonucleotide primers are grouped according to the identity of the first base which would be expected to be added to the primer through the process of primer extension; hybridizing to the oligonucleotide primers a sample nucleic acid sequence which possibly contains a mutation; extending each oligonucleotide primer by one base using a reaction mixture comprising labeled ddNTPs; and detecting a mutation in the sample nucleic acid sequence by detecting the presence of a labeled ddNTP which does not correspond to the identity of the base expected to be added to the primer through the process of primer extension. Mutations may be detected in mRNA or DNA.

Abstract: The present invention introduces a class of compounds, 2-substituted carbonyl compounds, that are useful as disinfecting agents, and particularly useful in methods for the disinfection of biological fluids. Members of the class of compounds have been found to possess pathogen inactivating properties against a variety of pathogens. The compounds and methods disclosed herein are useful for disinfecting biological fluids such as blood and blood fractions, among others.

Abstract: A method for adsorption of a substance from a liquid sample on a fluidized bead or stirred suspension, in which the beads used comprise a base matrix and exhibit a structure having affinity to the substance, characterized in that the structure is covalently bound to the base matrix via an extender. Populations of beads in which the beads contain a filler incorporated in a base matrix and an extender are also described.

Abstract: This invention is directed to a cassette chemical immobilization and treatment system and method for enabling the performance of various complex chemistries with minimal human intervention, near-zero dead volume, and flow-through protocols pursuant to a predetermined instruction set encoded on a multiple-address chemical treatment cassette assembly. The cassette assembly comprises a plurality of analyte sample columns (“mini-columns”), reagent wells containeing pre-packaged reagents including powdered, micoencapsulated, liquid or lyophillized reagents, analyte loading funnels, alignment means for the analyte sample columns, and, a machine readable instruction code Bet for identifying a chemical treatment protocol. The mini-columns are improved columns having high pressure interface capability to permit direct insertion of the mini-column into high-pressure solvent line for use as a support column for HPLC analysis.

Abstract: Process for, in layers of a porous matrix, exhibiting the groups A, introducing a functionality using a reagent I, introducing the functionality by a reaction with the groups A. The characterizing feature is that the matrix is contacted with a functional deficiency of reagent I and that conditions and reagent I are chosen so that the reaction between reagent I and the groups A is more rapid than diffusion of reagent I in the matrix.

Abstract: A polymer article having structure (I), where P comprises the basic polymer and groups —L—CH(—X(R1)p)—CH2(—Y(R2)p); L is a part of a pending group utilized for introducing —X(R1)p and —Y(R2)p; X and Y are halogen, N, S and O; R1 is hydrogen, alkyl, acyl or R2 when X is N, O or S; R2 is hydrogen, alkyl, alkylaryl or arylalkyl in which the alkyl part may contain 1-18 carbons, or —R3(—NH—CR4═O)q or —R3(—NH2)q, —CR4═O or poly alkyloxy that may have been terminally acylated or alkylated; p is an integer 0-3, with the provisos a) p depends on X being halogen, O, S or N, and b) that if several groups R2 are present they may be identical or different; R3 is alkyl, —O-alkyl, hydroxyalkyl, phenylalkyl, with up to 18 carbon atoms in the alkyl part, and R4 is C1-18 alkyl. q is an integer>O representing that one or more hydrogens in R3 may have been replaced with —NH2 or —NH—CR4═O.

Abstract: A process is provided for large scale purification of plasmid DNA to obtain plasmid DNA of pharmaceutical grade. Steps of the process include lysing cells such as by alkaline lysis to obtain a lysate containing plasmid DNA, chromosomal DNA and RNA, precipitating most of the RNA and Chromosomal DNA by adding divalent alkaline earth metal ions such as provided by calcium chloride, removing the precipitated RNA and chromosomal DNA, and contacting the lysate with an anion exchange matrix to obtain purified plasmid DNA. The anion exchange matrix may be cross-linked agarose containing anion exchange groups bound to the matrix via an extender such as dextran. The anion exchange groups may be quaternary ammonium groups. The purified plasmid DNA may be further contacted with an anion exchange matrix such as hydrophilyzed polystyrene/divinyl benzene substituted with quaternary ammonium groups to obtain purified plasmid DNA of gene therapy grade.

Abstract: The present invention relates generally to a detection device for a spectrophotometer system, and in particular to a light detection device with an analogue input and a digital output. The object of the present invention is to provide a more accurate detection device, which requires substantially less space for accommodating the detection device in a spectrophotometer. This object is solved by using a successive approximation A/D converter (16) which has internal sample and hold circuits, and which heretofore has been used in the audio industry. This will increase the accuracy of the detection device and make it more resistant against disturbances. It will also decrease the space required for such detection device, down to one third compared to conventional detection devices.

Abstract: A separation medium having a base matrix and matrix-bound groups which exhibit recombinant Protein A containing a cysteine. The groups are of formula: —B—X—rProtein A-cys where B is a bridge which binds to the base matrix and X includes a heteroatom N or S from rProtein A-cys. In a preferred embodiment X is a thioether sulphur and/or a secondary amine (—NH—). An alternative embodiment features a variant of Protein A in which the C-terminal residue is cysteine.

Abstract: Support beads for downstream processing including a polymer, esp. a polysaccharide, matrix into which glass or silica particles, preferably quartz particles, have been incorporated, and their use in downstream processing, especially stabilized fluidized bed separations.

Abstract: A method for primer walking cycle sequencing of nucleic acid is provided using a presynthesized set of walking primers wherein the primers have a raised annealing temperature and/or improved annealing properties.