Abstract: A method of analyzing a polynucleotide of interest, comprising providing one or more sets of consecutive oligonucleotide primers differing within each set by one base at the growing end therof; annealing a single strand of the polynucleotide or a fragment of the polynucleotide to the oligonucleotide primers under hybridization conditions; subjecting the primers to single base extension reactions with a polymerase and terminating nucleotides, the terminating nucleotides being mutually distinguishable; and observing the location and identity of each terminating nucleotide to thereby analyze the sequence or a part of the nucleotide sequence of the polynucleotide of interest, is disclosed. An apparatus comprising a solid support to which is attached at defined locations thereon one or more sets of consecutive oligonucleotide primers differing within each set by one base at the growing end thereof is also described.

Abstract: An access valve suitable for controlling fluid flow into and out of a chromatography column has a central axially movable probe with a head acting as a spool valve in a barrel. Axial movement of the probe adjusts the valve between a fully open condition, in which bota first conduit extending through the probe and a second conduit defined around the probe are open to the column interior, a partly open condition in which a second sealing land on the probe closes the second conduit, and a fully closed position in which both conduits are closed. The three positions are useful for packing and unpacking chromatography media into and from the column. In the closed condition of the valve, the first and second conduits communicate with one another so that the valve interior can be cleaned while the column is operating.

Abstract: A sliding valve having a flow line on which there may be placed a module having flow channel that can be connected and disconnected. The valve avoids the problem of liquid remaining in the flow channel of a first valve component by providing flow channel intersecting segment which intersects both components of the sliding valve so that in a module disconnecting orientation, each valve component defines separate segments of the flow channel while in the connected orientation, the valve components define a single flow channel. The construction allows for liquid flow through the valve independent of the module being in connected or disconnected position.

Abstract: A method for the separation of a predetermined compound comprising i) partitioning the compound in a two-phase (system A) in which at least one of the phases is rich (phase 1) and the other is poor (phase 2) in a thermoseparating polymer (I), ii) collecting one phase (phase 1 or phase 2) containing said compound, and iii if desired, further working up said compound from the phase collected in step ii, characterized a) in that polymer (I) is a micell-forming thermoseparating polymer, and b) in that phase 2 possibly contains at least one agent that is cloud point-decreasing for thermoseparating polymer (I), such as a polymer (II) that is incompatible with polymer (I) or a salt.

Abstract: A fraction collector (1) for dispensing liquid from a liquid source into receptacles (23) carried on a rotatable turntable (6), wherein the turntable is connected to the fraction collector casing (5) via a support arm (7) such that the turntable is rotatably mounted at one end of said support arm, and said support arm at its other end being rotatably mounted to the fraction collector casing. Thereby it is possible to align any position of the turntable under a dispensing tube (4) to dispense a liquid into a receptacle placed anywhere on the turntable.

Abstract: The present invention introduces a class of compounds, 2-substituted carbonyl compounds, that are useful as disinfecting agents, and particularly useful in methods for the disinfection of biological fluids. Members of the class of compounds have been found to possess pathogen inactivating properties against a variety of pathogens. The compounds and methods disclosed herein are useful for disinfecting biological fluids such as blood and blood fractions, among others.

Abstract: This invention is directed to a cassette chemical immobilization and treatment system and method for enabling the performance of various complex chemistries with minimal human intervention, near-zero dead volume, and flow-through protocols pursuant to a predetermined instruction set encoded on a multiple-address chemical treatment cassette assembly. The cassette assembly comprises a plurality of analyte sample columns (“mini-columns”), reagent wells containeing pre-packaged reagents including powdered, micoencapsulated, liquid or lyophillized reagents, analyte loading funnels, alignment means for the analyte sample columns, and, a machine readable instruction code Bet for identifying a chemical treatment protocol. The mini-columns are improved columns having high pressure interface capability to permit direct insertion of the mini-column into high-pressure solvent line for use as a support column for HPLC analysis.

Abstract: A method for adsorption of a substance from a liquid sample on a fluidized bead or stirred suspension, in which the beads used comprise a base matrix and exhibit a structure having affinity to the substance, characterized in that the structure is covalently bound to the base matrix via an extender. Populations of beads in which the beads contain a filler incorporated in a base matrix and an extender are also described.

Abstract: Process for, in layers of a porous matrix, exhibiting the groups A, introducing a functionality using a reagent I, introducing the functionality by a reaction with the groups A. The characterizing feature is that the matrix is contacted with a functional deficiency of reagent I and that conditions and reagent I are chosen so that the reaction between reagent I and the groups A is more rapid than diffusion of reagent I in the matrix.

Abstract: A polymer article having structure (I), where P comprises the basic polymer and groups —L—CH(—X(R1)p)—CH2(—Y(R2)p); L is a part of a pending group utilized for introducing —X(R1)p and —Y(R2)p; X and Y are halogen, N, S and O; R1 is hydrogen, alkyl, acyl or R2 when X is N, O or S; R2 is hydrogen, alkyl, alkylaryl or arylalkyl in which the alkyl part may contain 1-18 carbons, or —R3(—NH—CR4═O)q or —R3(—NH2)q, —CR4═O or poly alkyloxy that may have been terminally acylated or alkylated; p is an integer 0-3, with the provisos a) p depends on X being halogen, O, S or N, and b) that if several groups R2 are present they may be identical or different; R3 is alkyl, —O-alkyl, hydroxyalkyl, phenylalkyl, with up to 18 carbon atoms in the alkyl part, and R4 is C1-18 alkyl. q is an integer>O representing that one or more hydrogens in R3 may have been replaced with —NH2 or —NH—CR4═O.

Abstract: The present invention relates generally to a detection device for a spectrophotometer system, and in particular to a light detection device with an analogue input and a digital output. The object of the present invention is to provide a more accurate detection device, which requires substantially less space for accommodating the detection device in a spectrophotometer. This object is solved by using a successive approximation A/D converter (16) which has internal sample and hold circuits, and which heretofore has been used in the audio industry. This will increase the accuracy of the detection device and make it more resistant against disturbances. It will also decrease the space required for such detection device, down to one third compared to conventional detection devices.

Abstract: A process is provided for large scale purification of plasmid DNA to obtain plasmid DNA of pharmaceutical grade. Steps of the process include lysing cells such as by alkaline lysis to obtain a lysate containing plasmid DNA, chromosomal DNA and RNA, precipitating most of the RNA and Chromosomal DNA by adding divalent alkaline earth metal ions such as provided by calcium chloride, removing the precipitated RNA and chromosomal DNA, and contacting the lysate with an anion exchange matrix to obtain purified plasmid DNA. The anion exchange matrix may be cross-linked agarose containing anion exchange groups bound to the matrix via an extender such as dextran. The anion exchange groups may be quaternary ammonium groups. The purified plasmid DNA may be further contacted with an anion exchange matrix such as hydrophilyzed polystyrene/divinyl benzene substituted with quaternary ammonium groups to obtain purified plasmid DNA of gene therapy grade.

Abstract: A separation medium having a base matrix and matrix-bound groups which exhibit recombinant Protein A containing a cysteine. The groups are of formula: —B—X—rProtein A-cys where B is a bridge which binds to the base matrix and X includes a heteroatom N or S from rProtein A-cys. In a preferred embodiment X is a thioether sulphur and/or a secondary amine (—NH—). An alternative embodiment features a variant of Protein A in which the C-terminal residue is cysteine.

Abstract: The invention concerns a cassette for an electrophoresis system including an essentially plate shaped body, which includes a first wall for supporting a gel plate, and a second wall for supporting buffer strips, the walls being connected to each other. Further, the second wall is displaceable with respect to the first wall between a first inactive position where the buffer strips are chemically and electrically separated from the gel plate and a second, active position where the buffer strips are in electrical and chemical contact with the gel plate.

Abstract: A method of analyzing a sequence of a polynucleotide of interest, comprising the steps of: a) incorporating one member of a specific binding pair at the end of each strand of a double stranded polynucleotide of interest, the number being of the same type for both strands, b) immobilizing both strands of the polynucleotide to a solid support provided with the other member of the specific binding pair, c) annealing sequencing primers to the immobilized strands, d) sequencing both strands by the chain termination method. The polynucleotide of interest is preferably amplified before or in connection with step a) and most preferably by polymerase chain reaction extension. The invention also comprises a kit for use in analyzing the sequence of a polynucleotide of interest.

Abstract: Support beads for downstream processing comprising a polymer, esp. a polysaccharide, matrix into which glass or silica particles, preferably quartz particles, have been incorporated, and their use in downstream processing, especially stabilized fluidized bed separations.

Abstract: The use of a gel produced by polymerization of a dextran derivative which exhibits groups that contain an alkene structure, for separating nucleic acid by electrophoresis.

Abstract: The invention relates to a pump system for providing and maintaining a controlled outflow, comprising a couple of piston pump units (1a, 1b), having at least two cylinders (2a, 2b, 2c, 2d) each with a piston (3a, 3b, 3c, 3d) slidably arranged in each cylinder. It further comprises devices for controllably moving the piston in each cylinder, namely by an eccentric wheel (15a, 15b, 15c, 15d) for each of the pistons, acting on one end thereof. There is also provided a control unit for dynamically changing the speed of movement of the devices for moving the piston, in response to pressure changes in the system on the pressure side, for maintaining a desired flow out of the pump.

Abstract: A chromatographic method and device for separating one or several organic substances in a liquid sample. According to the method said sample of substances is passed through a chromatographic device containing as separation medium at least one continuous macroporous matrix. The macroporous matrix comprises a cross-linked organic polymer prepared by polymerization of a high internal phase emulsion system of a water-in-oil emulsion of organic monomers, said emulsion containing at least 75% by weight of water phase. The polymerization results in an open porous structure, to allow a convective flow to pass through the macroporous matrix, whereby said organic substances separate from each other and/or the liquid. The pores of the macroporous matrix are unmodified, or surface modified in a manner that the convective flow is not hampered.

Abstract: In a method for determining the existence of a mutation in a nucleic acid fragment from an electrical signal generated by a DNA sequencer made up of sequence information produced by fluorescent fragment products of different lengths of said nucleic acid fragment, followed by a run-off peak produced by fluorescent full length fragment products of said nucleic acid fragment, any difference between said run-off peak and a reference run-off peak generated by unmutated full length fragment products of said nucleic acid fragment, is determined, a difference indicating the existence of a deletion or insertion mutation.