Abstract: Novel pyridine compounds having the formula R.sup.1 -S-S-A-Z are disclosed, in which formula R.sup.1 is 2-pyridyl, 5-nitro-2-pyridyl or 4-pyridyl, A is a hydrocarbon residue having 1-10 carbon atoms and Z is a group ##STR1## or acid addition salts of the last mentioned group, where n is 2 or 3, R.sup.1 has the same significance as R.sup.1 above and is equal thereto and R.sup.2 is methyl or ethyl. These compounds are particularly useful as bifunctional coupling agents and as thiolating agents.

Abstract: The invention relates to a method of coupling organic ligands to a polymeric carrier. The organic ligand contains at least one primary or secondary amino group, at least one thiol group and/or at least one aromatic hydroxy group, and the polymeric carrier contains at least one hydroxy group. According to the invention the coupling is performed by forming a reactive derivative of the polymeric carrier by treatment with a sulfonyl halogenid, and reacting the formed reactive derivative with the organic ligand, which is thereby bonded directly to a carbon atom in the polymeric carrier. The polymeric carrier can, for example, be a possibly cross-linked polysaccharide, and the organic ligand is preferably a biological material such as a protein. The coupling product can, for example, be used for immunologic determinations, for affinity chromatography, etc. The coupling method can be performed under mild conditions not damaging sensitive organic ligands.

Abstract: A method and a device for applying a sample solution to a liquid chromatographic column. The device comprises a container of uniform cross section and a piston slidably arranged in said container and sealingly contacting the container wall so as to divide the container into a driving solution chamber and a sample solution chamber. The piston is arranged to be displaced towards the sample solution chamber when driving solution is pumped into the driving solution chamber while expelling sample solution from the sample solution chamber in an amount proportional to the amount of driving solution pumped into the driving solution chamber. When all sample solution has been expelled from the sample solution chamber the driving solution chamber is automatically put into communication with the outlet of the container.

Abstract: A water-soluble ampholyte which comprises the reaction product between(a) one or more compounds selected from the group comprising certain amino-acids, hydroxylamine, an amine of the formula ##STR1## wherein R.sub.1 is hydrogen, the group H.sub.2 N--(CH.sub.2).sub.n --, wherein n is an integer 2-6, or the group H.sub.2 N--(CH.sub.2).sub.3 --NH--(CH.sub.2).sub.3 --, R.sub.2 and R.sub.3 are the same or different and each representing hydrogen, methyl, ethyl or hydroxyethyl or together with the intermediate nitrogen atom form certain heterocyclic groups, and certain di- and tripeptides, at least one of these compounds being one which contains at least one carboxyl group, or salts of the aforementioned compounds, and(b) at least one bifunctional alkylating agent containing a straight or branched alkylene chain having 2-10 carbon atoms and which chain may be substituted with certain substituents and/or be broken by 1-3 oxygen atoms with certain conditions, in a molar relationship between (b) and (a) of 1:2-9:10.

Abstract: A medium for isoelectric focusing is produced which consists of or contains agarose into which positively charged substituents have been introduced to neutralize negatively charged groups inherently present in the agarose, said substituents containing as the only charged group a quaternary amino group with a positive charge independent of pH at least in the range 2-12. The medium may consist of a mixture of two or more agarose preparations with different degrees of substitution with regard to the introduced substituents. Unmodified agarose may also constitute one of the components of the medium.

Abstract: A novel class of reactive derivatives of polymers containing HS-groups is disclosed in which derivatives a number of the HS-groups are converted to a disulphide group of the formula -S-S-A-Z, where A is a hydrocarbon residue having 1-10 carbon atoms and Z is a group ##STR1## or acid addition salts of the last mentioned group, where n is 2 or 3, R.sup.1 is 2-pyridyl, 5-nitro-2-pyridyl or 4-pyridyl and R.sup.2 is methyl or ethyl. The novel derivatives may be used, for example, as thiolating agents or as carriers for e.g. enzymes or other proteins etc.

Abstract: Novel pyridine compounds having the formula R.sup.1 -S-S-A-Z are disclosed, in which formula R.sup.1 is 2-pyridyl, 5-nitro-2-pyridyl or 4-pyridyl, A is a hydrocarbon residue having 1-10 carbon atoms and Z is a group ##STR1## or acid addition salts of the last mentioned group, where n is 2 or 3, R.sup.1 has the same significance as R.sup.1 above and is equal thereto and R.sup.2 is methyl or ethyl. These compounds are particularly useful as bifunctional coupling agents and as thiolating agents.

Abstract: A separation column comprising a column tube and terminal flanges, wherein the terminal flanges are attached to the column tube by means of a two-layer material which encloses a portion of the column tube and a portion of one of said terminal flanges, said two-layer material comprising a fusible plastic layer in contact with the column tube and said terminal flange and an outer hose of shrinking plastic, having been subjected to a heat treatment causing the fusible plastic layer to fuse and the shrinking plastic hose to shrink.

Abstract: The present invention relates to an agent for intravascular administration for diagnostic and/or physiologic investigations, which agent consists of or contains a suspension of minute radioactive particles in a physiologically acceptable liquid.Radioactively labelled microspheres, in suspension in a physiologically acceptable liquid, have lately been increasingly used in circulation studies, studies of the shunting phenomena, microcirculation studies, nutrition studies and as a means for carrying out scintography on man and animals.

Abstract: In optical analysis of fluids a method and an apparatus are described by which it is possible to continuously measure the concentration value of the fluid within a very broad range of concentration values. In one single test glass optical paths of different lengths are provided substantially perpendicularly to each other. The concentration value of the fluid is measured simultaneously along the two mutually perpendicular optical paths.

Abstract: A dextran derivative gel in particle form for use in the separation of substances according to their molecular size and/or electrical charge by means of gel-chromatographic and/or ion exchange techniques is disclosed which gel comprises a copolymerizate obtained by the free-radical copolymerization of a dextran derivative containing vinyl and/or substituted vinyl groups with (a) a divinyl compound of the formula ##STR1## wherein R' and R" are equal or different and each represent hydrogen or methyl and X is ##STR2## --SO.sub.2 -- or the group ##STR3## wherein n is an integer from 1 to 6, inclusive, and optionally (b) a low-molecular weight monovinyl compound.

Abstract: A dextran derivative gel for use in electrophoretic separation processes is disclosed, which gel comprises a polymerizate obtained by the free-radical homopolymerization of a dextran derivative containing vinylic groups of the formula ##STR1## wherein A is --CH.sub.2 -- or --O-- and R is a hydrogen atom, a methyl group, a trifluoromethyl group, a fluorine, chlorine or bromine atom or a cyano group, or free-radical copolymerization of such a vinylic derivative of dextran with a low-molecular weight monovinyl compound.

Abstract: This invention relates to a new method of isolating highly purified albumin in high yields from plasma products.According to the invention a particular plasma fraction, containing albumin in dissolved form and being essentially free from the coagulation factors I, II, VII, VIII, IX and X and from the main part of IgG, is subjected to a two-step chromatographic separation using aqueous buffer systems. The first step is carried out on an anion exchanger and the second step on a cation exchanger, or vice versa. In a preferred embodiment a buffer of pH 4.5 - 4.9 and an ionic strength of 0.025 - 0.1 is used for the separation on the anion exchanger, whereas a buffer of pH 5.2 - 6.5 and an ionic strength of 0.1 - 0.05 is used for the separation on the cation exchanger.