Abstract: This invention relates to a process for selective capture, isolation and purification of chlorinated sucrose compounds, including chlorinated sucrose, their precursors and derivatives, including trichlorogalactosucrose (TGS), directly from chlorinated reaction mixture by column chromatography on adsorbents and under conditions which result in specific and selective affinity towards one or more of chlorinated sucrose compound. The process also integrates de-esterification of chlorinated sucrose esters adsorbed on the adsorbent while they are being treated with desorbent. The process also provides a novel approach to concentration and crystallization of TGS. The chlorinated sucrose derivatives, including TGS, thus isolated are substantially free from most impurities, salts and organic solvents. The process has high recovery of more than 95% in terms of desired chlorinated sucrose derivatives including TGS.

Abstract: A process is described which uses whole cell preparations, immobilized or without immobilization, of a microorganism, including Aureobasidium pullulans, capable of forming enzymes of the group of fructosyltransferases, for catalyzing a reaction between sucrose and 6-O-protected glucose for formation of 6-O-protected sucrose, an intermediate in synthesis of trichlorogalactosucrose. 6-O-protected Sucrose is separated from high molecular weight by-products of the reaction having molecular weight of 500 daltons and more by reverse osmosis, and further purified by column chromatography.

Abstract: A process of azeotropic removal of dimethylformamide, abbreviated as DMF, from a process stream containing DMF requiring its removal, is described wherein the said Process Stream being obtained in a process for preparation of 4,1?,6? trichlorogalactosucrose, abbreviated as TGS, or TGS-6-ester including TGS-6-acetate or TGS-6-benzoate, comprising steps of (a) evaporation of the said process stream under reduced pressure to a concentrate to effect removal of a part of DMF azeotropically, (b) diluting the concentrate obtained at the end of step (a.) of this claim with water, preferably to about 5 to 10 times the volume of the said concentrate, and (c) repeating the cycles of evaporation under reduced pressure and dilution with water for more number of times until content of DMF in the concentrated mass is reduced to 0.5% or less of the concentrate.