Patents Assigned to Pharming B.V.
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Publication number: 20050198698Abstract: Transgenes for producing recombinant polypeptides transgenic bovine species. A transgene for producing recombinant polypeptides in the milk of transgenic bovine species comprises at least one expression regulation sequence, a secretory DNA sequence encoding a secretory signal sequence which is functional in mammary secretory cells of the bovine species and a recombinant DNA sequence encoding the recombinant polypeptide. Also included are methods for producing transgenic bovine species. The method includes introducing the above transgene into an embryonal target cell of a bovine species, transplanting the transgenic embryonic target cell formed thereby into a recipient bovine parent and identifying at least one female offspring which is capable of producing the recombinant polypeptide in its milk.Type: ApplicationFiled: November 12, 2004Publication date: September 8, 2005Applicant: Pharming B.V., a Netherlands corporationInventors: Herman DeBoer, Rein Strijker, Herbert Heyneker, Gerard Plantenburg, Sang Lee, Frank Pieper, Paul Krimpenfort
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Publication number: 20050172342Abstract: The invention provides transgenic nonhuman mammals capable secreting exogenous procollagen or collagen into their milk. The mammals are healthy and capable of producing procollagen or collagen at high levels, usually in trimeric form. Suitable transgenes for incorporation into the mammals are also provided.Type: ApplicationFiled: December 18, 2003Publication date: August 4, 2005Applicants: Pharming B.V., Cohesion Technologies, Inc.Inventors: Costas Karatzas, Frank Pieper, Ineke De Wit, Richard Berg, Gerard Platenburg, Paul Toman
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Publication number: 20040266711Abstract: Described is a method of targeting specific genes to the mammary gland which results in the efficient synthesis and secretion of biologically important molecules. Further, there is described as a composition of matter, a transgenic mammal having the ability to reproduce itself and being suitable for the secretion of biologically active agents into its milk. Additionally there is disclosed as a composition of matter, recombinant DNA gene complexes designed to integrate into a mammalian genome and to synthesize and secrete biological active agents into the milk. Furthermore, methods of producing and using altered milk are disclosed.Type: ApplicationFiled: March 3, 2004Publication date: December 30, 2004Applicant: Pharming B.V.Inventor: Jeffrey M. Rosen
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Publication number: 20040250300Abstract: This invention relates to the production of recombinant proteins in mammals' milk. Particularly, this invention relates to an expression system which when transgenically incorporated into a mammal permits the female species of that mammal to produce the desired recombinant protein in or along with its milk. This invention also relates to the transgenic mammal that produces the desired recombinant product in its milk.Type: ApplicationFiled: March 2, 2004Publication date: December 9, 2004Applicant: Pharming B.V.Inventors: Harry Meade, Nils Lonberg
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Publication number: 20040003421Abstract: The invention provides transgenic nonhuman mammals producing phosphorylated lysosomal proteins in their milk, and methods of generating the same. Phosphorylation occurs at the 6′ position of a mannose side chain residue. Also provided are methods of purifying lysosomal proteins from milk, and incorporating the proteins into pharmaceutical compositions for use in enzyme replacement therapy.Type: ApplicationFiled: January 23, 2003Publication date: January 1, 2004Applicant: Pharming B.V.Inventors: Arnold J.J. Reuser, Ans T. Van der Ploeg, Frank R. Pieper, Martin Ph. Verbeet
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Publication number: 20030192068Abstract: Transgenes for producing recombinant polypeptides transgenic bovine species. A transgene for producing recombinant polypeptides in the milk of transgenic bovine species comprises at least one expression regulation sequence, a secretory DNA sequence encoding a secretory signal sequence which is functional in mammary secretory cells of the bovine species and a recombinant DNA sequence encoding the recombinant polypeptide. Also included are methods for producing transgenic bovine species. The method includes introducing the above transgene into an embryonal target cell of a bovine species, transplanting the transgenic embryonic target cell formed thereby into a recipient bovine parent and identifying at least one female offspring which is capable of producing the recombinant polypeptide in its milk.Type: ApplicationFiled: June 11, 2002Publication date: October 9, 2003Applicant: Pharming B.V.Inventors: Herman A. DeBoer, Rein Strijker, Herbert L. Heynecker, Gerard Platenburg, Sang Ha Lee, Frank Pieper, Paul J.A. Krimpenfort
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Patent number: 6140552Abstract: Transgenes for producing recombinant polypeptides transgenic bovine species. A transgene for producing recombinant polypeptides in the milk of transgenic bovine species comprises at least one expression regulation sequence, a secretory DNA sequence encoding a secretory signal sequence which is functional in mammary secretory cells of the bovine species and a recombinant DNA sequence encoding the recombinant polypeptide. Also included are methods for producing transgenic bovine species. The method includes introducing the above transgene into an embryonal target cell of a bovine species, transplanting the transgenic embryonic target cell formed thereby into a recipient bovine parent and identifying at least one female offspring which is capable of producing the recombinant polypeptide in its milk.Type: GrantFiled: June 7, 1995Date of Patent: October 31, 2000Assignee: Pharming B.V.Inventors: Herman A. Deboer, Rein Strijker, Herbert L. Heyneker, Gerard Platenburg, Sang He Lee, Frank Pieper, Paul J. A. Krimpenfort
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Patent number: 6118045Abstract: The invention provides transgenic nonhuman mammals producing phosphorylated lysosomal proteins in their milk, and methods of generating the same. Phosphorylation occurs at the 6' position of a mannose side chain residue. Also provided are methods of purifying lysosomal proteins from milk, and incorporating the proteins into pharmaceutical compositions for use in enzyme replacement therapy.Type: GrantFiled: July 29, 1996Date of Patent: September 12, 2000Assignees: Pharming B.V., The Universiteit Leiden, Academic Hospital, Eramus UniversiteitInventors: Arnold J. J. Reuser, Ans T. Van der Ploeg, Frank R. Pieper, Martin Ph. Verbeet
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Patent number: 6066725Abstract: Transgenes for producing recombinant polypeptides transgenic bovine species are described. A transgene for producing recombinant polypeptides in the milk of transgenic bovine species comprises at least one expression regulation sequence, a secretory DNA sequence encoding a secretory signal sequence which is functional in mammary secretory cells of the bovine species and a recombinant DNA sequence encoding the recombinant polypeptide. Also included are methods for producing transgenic bovine species. The method includes introducing the above transgene into an embryonal target cell of a bovine species, transplanting the transgenic embryonic target cell formed thereby into a recipient bovine parent and identifying at least one female offspring which is capable of producing the recombinant polypeptide in its milk.Type: GrantFiled: September 21, 1998Date of Patent: May 23, 2000Assignee: Pharming B.V.Inventors: Herman A. DeBoer, Rein Strijker, Herbert L. Heyneker, Gerard Platenburg, Sang He Lee, Frank Pieper, Paul J. A. Krimpenfort
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Patent number: 6013857Abstract: Transgenes for producing recombinant polypeptides transgenic bovine species. A transgene for producing recombinant polypeptides in the milk of transgenic bovine species comprises at least one expression regulation sequence, a secretory DNA sequence encoding a secretory signal sequence which is functional in mammary secretory cells of the bovine species and a recombinant DNA sequence encoding the recombinant polypeptide. Also included are methods for producing transgenic bovine species. The method includes introducing the above transgene into an embryonal target cell of a bovine species, transplanting the transgenic embryonic target cell formed thereby into a recipient bovine parent and identifying at least one female offspring which is capable of producing the recombinant polypeptide in its milk.Type: GrantFiled: June 5, 1995Date of Patent: January 11, 2000Assignee: Pharming B.V.Inventors: Herman A. Deboer, Rein Strijker, Herbert L. Heyneker, Gerard Platenburg, Sang He Lee, Frank Pieper, Paul J. A. Krimpenfort
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Patent number: 5994616Abstract: Described are methods of targeting specific genes or DNA sequences encoding biologically active proteins to the mammary gland such that the DNA sequence is expressed in the mammary gland and the protein is secreted into the milk of the mammal. Also described is the production of transgenic nonhuman mammals whose germ line comprises a DNA gene complex containing a casein promoter, a casein enhancer, a DNA sequence which encodes a signal peptide and a DNA sequence encoding a biologically active protein. The milk containing the biologically active protein or the biologically active protein itself can be used in the making of diary products.Type: GrantFiled: January 25, 1994Date of Patent: November 30, 1999Assignee: Pharming B.V.Inventor: Jeffrey M. Rosen
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Patent number: 5959171Abstract: A process is disclosed for the production of a protein or polypeptide in the milk of a transgenic non-human mammal where the protein or polypeptide is produced as fusion protein with another protein. The fusion protein can then be cleaved to release the protein or polypeptide. This method would reduce or prevent the formation of side effects associated with ectopic expression or leakage of the protein or polypeptide. Such a fusion protein is .beta.-lactoglobulin-EPO, where biologically active EPO is released upon cleavage of the fusion protein.Type: GrantFiled: August 17, 1994Date of Patent: September 28, 1999Assignee: Pharming B.V.Inventors: Juha-Matti Hyttinen, Veli-Pekka Korhonen, Juhani Janne
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Patent number: 5919913Abstract: The invention provides methods for purification of human lactoferrin from milk, especially milk of nonhuman species, and for separation of human lactoferrin from undesired macromolecular species present in the milk, including separation from nonhuman lactoferrin species.Type: GrantFiled: March 9, 1995Date of Patent: July 6, 1999Assignee: Pharming B.V.Inventors: Jan H. Nuyens, Harry H. Van Veen
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Patent number: 5861491Abstract: The invention provides methods for purification of human lactoferrin from milk, especially milk of nonhuman species, and for separation of human lactoferrin from undesired macromolecular species present in the milk, including separation from nonhuman lactoferrin species.Type: GrantFiled: October 16, 1996Date of Patent: January 19, 1999Assignee: Pharming B.V.Inventors: Jan H Nuijens, Harry H Van Veen
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Patent number: 5750172Abstract: This invention relates to the production of recombinant proteins, such as coagulation factors VIII and IX, tissue plasminogen activator (TPA), urokinase, growth hormone, insulin, interferons, interleukins, peptide hormones and immunoglobulins, in mammals' milk. Particularly, this invention relates to an expression system which when transgenically incorporated into a mammal permits the female species of that mammal to produce the desired recombinant protein in or along with its milk. This invention also relates to the transgenic mammal that produces the desired recombinant product in its milk.Type: GrantFiled: June 5, 1995Date of Patent: May 12, 1998Assignee: Pharming B.V.Inventors: Harry Meade, Nils Lonberg
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Patent number: 5741957Abstract: A transgenic bovine is disclosed whose somatic and germ cells contain a transgene, wherein the transgene comprising a mammary gland specific promoter, a mammary gland specific enhancer, a DNA sequence encoding a signal sequence functional in bovine mammary gland secretory cells and a DNA sequence encoding a heterologous polypeptide of interest wherein the transgenic bovine expresses the transgene such that the polypeptide of interest is detectable in milk produced by the transgenic bovine.Type: GrantFiled: June 5, 1995Date of Patent: April 21, 1998Assignee: Pharming B.V.Inventors: Herman A. Deboer, Rein Strijker, Herbert L. Heyneker, Gerard Platenburg, Sang He Lee, Frank Pieper, Paul J. A. Krimpenfort
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Patent number: 5721367Abstract: The invention relates to methods for intracellularly producing DNA segments by homologous recombination of smaller overlapping DNA fragments and transgenic mammalian cells and transgenic non-human mammals produced by such methods.Type: GrantFiled: June 5, 1995Date of Patent: February 24, 1998Assignee: Pharming B.V.Inventors: Robert M. Kay, Anton Berns, Paul Krimpenfort, Frank Pieper, Rein Strijker
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Patent number: 5565362Abstract: Described is a method of targeting specific genes to the mammary gland which results in the efficient synthesis and secretion of biologically important molecules. Further, there is described as a composition of matter, a transgenic mammal having the ability to reproduce itself and being suitable for the secretion of biologically active agents into its milk. Additionally there is disclosed as a composition of matter, recombinant DNA gene complexes designed to integrate into a mammalian genome and to synthesize and secrete biological active agents into the milk. Furthermore methods of producing and using altered milk are disclosed.Type: GrantFiled: January 24, 1994Date of Patent: October 15, 1996Assignee: Pharming B.V.Inventor: Jeffrey M. Rosen