Abstract: Apparatuses and methods are described for the use of optically driven bubble, convective and displacing fluidic flow to provide motive force in microfluidic devices. Alternative motive modalities are useful to selectively dislodge and displace micro-objects, including biological cells, from a variety of locations within the enclosure of a microfluidic device.
Type:
Grant
Filed:
October 12, 2020
Date of Patent:
October 31, 2023
Assignee:
PHENOMEX INC.
Inventors:
Volker L. S. Kurz, Troy A. Lionberger, Eric K. Sackmann, Kai W. Szeto, Paul M. Lebel, Brandon R. Bruhn, Keith J. Breinlinger, Eric D. Hobbs, Andrew W. McFarland, J. Tanner Nevill, Xiaohua Wang
Abstract: Methods, systems and kits are described herein for detecting the results of an assay. In particular, the methods, systems and devices of the present disclosure rely on a difference between the diffusion rates of a reporter molecule and an analyte of interest in order to quantify an amount of analyte in a microfluidic device. The analyte may be a secreted product of a biological micro-object.
Type:
Grant
Filed:
April 15, 2020
Date of Patent:
October 17, 2023
Assignee:
PHENOMEX INC.
Inventors:
Troy A. Lionberger, Phillip J. M. Elms, Anupam Singhal, Randall D. Lowe, Jr., Volker L. S. Kurz, Paul M. Lebel