Abstract: The present invention relates to the field of biochemistry, specifically to the bioproduction of glycolate. Host cells, especially cyanobacteria of the genus Synechocystis, are modified in several ways to increase extracellular glycolate, including: mutant Rubisco enzymes, overexpression of phosphoribulokinase (PRK) or phosphoglycolate phosphatase (PGP), a permease to export glycolate, like GIcA, or by reduction of the capacity to metabolize glycolate due to reduced or eliminated glycolate dehydrogenase, glycolate oxidase activity and/or lactate dehydrogenase.
Type:
Application
Filed:
January 24, 2020
Publication date:
March 31, 2022
Applicant:
Photanol B.V.
Inventors:
Aniek Doreen van der Woude, Klaas Jan Hellingwerf, Koen Mulder
Abstract: A process of producing an organic compound and/or an intermediary compound by feeding carbon dioxide to a culture of Cyanobacteria cells and subjecting the culture to light, wherein the cells are capable of expressing a nucleic acid molecule that confers the ability to convert a glycolytic intermediate into said organic/intermediary compound. The expression of the nucleic acid molecule is under the control of a regulatory system which responds to a change in the concentration of a nutrient in the culture.
Type:
Grant
Filed:
December 2, 2015
Date of Patent:
November 20, 2018
Assignee:
PHOTANOL B.V.
Inventors:
Klaas Jan Hellingwerf, Maarten Joost Teixeira de Mattos
Abstract: A process of producing an organic compound and/or an intermediary compound as defined herein by feeding carbon dioxide to a culture of a cyanobacterial cell and subjecting the culture to light, wherein the cell is capable of expressing a nucleic acid molecule, wherein the expression of the nucleic acid molecule confer on the cell the ability to convert a glycolytic intermediate into the organic compound and/or into the intermediary compound and wherein the nucleic acid molecule is under the control of a regulatory system which responds to a change in the concentration of a nutrient in the culture.
Type:
Grant
Filed:
December 17, 2008
Date of Patent:
January 5, 2016
Assignee:
PHOTANOL B.V.
Inventors:
Klaas Jan Hellingwerf, Maarten Joost Teixeira De Mattos
Abstract: A process of producing L-lactate as defined herein by feeding carbon dioxide to a culture of a cyanobacterial cell and subjecting said culture to light, wherein said cell is capable of expressing a nucleic acid molecule, wherein the expression of said nucleic acid molecule confer on the cell the ability to convert a glycolytic intermediate into L-lactate and wherein said nucleic acid molecule is under the control of a regulatory system which responds to light or to a change in the concentration of a nutrient in said culture.
Type:
Application
Filed:
April 28, 2010
Publication date:
March 21, 2013
Applicant:
PHOTANOL B.V.
Inventors:
Martijn Bekker, Maarten Joost Teixeira de Mattos, Klaas Jan Hellingwerf