Abstract: A method of preparing a bioactive polypeptide in a stable, inactivated form, the method comprising the step of treating the polypeptide with ozonated water in order to oxidize and/or stabilize the cysteine residues, and in turn, prevent the formation of disulfide bridges necessary for bioactivity. The method can involve the use of ozonated water to both oxidize the disulfide bridges in a bioactive polypeptide, and to then stabilize the resultant cysteine residues. Optionally, and preferably, the method can involve the use of ozonated water to stabilize the cysteine residues, and thereby prevent the formation of disulfide bridges, in a polypeptide produced by recombinant means in a manner that allows the polypeptide to be recovered with the disulfide bridges unformed.

Abstract: An ointment base useful for preparing topically applied medicament formulations. The base can be provided in the form of a stable emulsion including paraffin(s), polyol, beeswax, cetostearyl alcohol, a 4-hydroxy benzoic acid lower alkyl ester, a surface active agent and a dispersing agent. The base can be combined with large volumes containing a variety of medicaments to form stable, useful formulations.