Abstract: A method for the regeneration of a cotton plant from somatic cells. The method comprises providing a cotton explant, culturing the explant in a callus growth medium supplemented with glucose as a primary carbon source until the secretion of phenolic compounds has ceased and undifferentiated callus is formed from the explant and culturing the undifferentiated callus in callus growth medium supplemented with sucrose as a primary carbon source until embryogenic callus is formed from the callus.
Type:
Grant
Filed:
June 7, 1995
Date of Patent:
December 10, 1996
Assignee:
Phytogen
Inventors:
Thirumale S. Rangan, Kanniah Rajasekaran
Abstract: There are provided methods for regenerating cotton by tissue and suspension culture starting with explants which are the hypocotyl, cotyledon or immature embryos. This also taught methods to transform cotton and improve cotton by selective growth.
Abstract: There are provided herewith vector precursors and vectors based on Pst I cleaved left and right border fragments of the T region of the Ti plasmid Agrobacterium tumefaciens strain of C58 interligated and ligated into the Pst I site of plasmid pBR322. Antibiotic resistance gene sequence is used to establish vector expression and presence in host cells.
Abstract: Mung bean sprouts of increased diameter are grown in an atmosphere containing oxygen and from about 0.5 to about 3 ppmv of a plant-growth regulator of ethylene, acetylene or mixtures thereof, and under a spray of an aqueous solution containing from about 0.1 to about 5 ppm weight-to-volume of a plant-growth hormone, which is 2,4-D, an auxin, or mixtures thereof.