Abstract: Method of genetic analysis of single cell samples comprising the steps of a) providing at least one single cell sample contained in a microplate comprising at least 5 wells/cm2, wherein each single cell sample is contained in an individual well of said microplate, said single cell sample being selected from a single cell and cells expanded from one single cell; b) lysing each of said at least one single cell sample in each of said individual well(s) to obtain DNA from said at least one single cell sample; c) amplifying a genetically relevant portion of said DNA by performing a polymerase chain reaction (PCR) to obtain an amplified PCR-product in each of said individual well(s); and d) analyzing said amplified PCR-product in each of said individual well(s).
Type:
Application
Filed:
October 9, 2009
Publication date:
September 29, 2011
Applicant:
PICOVITRO AB
Inventors:
Hélène Andersson Svahn, Ahmadian Afshin, Sara Lindström
Abstract: A method of cell seeding is provided, which comprises the steps of: providing a cell sample; seeding at least one cell of said cell sample into at least one well of a microplate comprising at least 5 wells/cm2. Also provided is a method of cell cultivation comprising the steps of: seeding at least one cell into a microplate using the method of cell seeding; incubating the microplate; and analyzing the contents of at least one well of the microplate.