Patents Assigned to Pierce Milwaukee, L.L.C.
  • METHOD FOR ISOLATION, AMPLIFICATION AND QUANTITATION OF RIBONUCLEIC ACID
    Publication number: 20070072229
    Abstract: A cellular RNA isolation method comprising treating at least one nucleated cell with a composition comprising mild detergents such as non-ionic detergent(s), buffer(s), chelator(s), reducing agent(s), salt(s), and RNase inhibitor(s) to result in a cytoplasmic lysate and intact nucleus, then separating the cytoplasmic lysate containing RNA from the nucleus, and using the cytoplasmic lysate for RNA analysis without further purifying the RNA.
    Type: Application
    Filed: September 28, 2006
    Publication date: March 29, 2007
    Applicant: Pierce Milwaukee, L.L.C.
    Inventors: Carolyn Bialozynski, Christopher Rosenau, T.S. Subramanian