Abstract: The present disclosure provides a set of spiked matrix standards comprising: one or more carriers, each carrier comprising: (a) a reference standard analyte in an amount that is different in each carrier; and (b) a blank matrix that is in admixture or in contact with the reference standard analyte. Methods for preparing and quantitating an analyte in a test sample comprises (a) providing one or more test samples to be tested for the presence of the analyte (b) providing a set of spiked matrix standards; (c) processing the standards and test samples and (d) quantitating the amount of analyte in each of the standards and test samples.

Abstract: The present disclosure provides a set of spiked matrix standards comprising: one or more carriers, each carrier comprising: (a) a reference standard analyte in an amount that is different in each carrier; and (b) a blank matrix that is in admixture or in contact with the reference standard analyte. Methods for preparing and quantitating an analyte in a test sample comprises (a) providing one or more test samples to be tested for the presence of the analyte (b) providing a set of spiked matrix standards; (c) processing the standards and test samples and (d) quantitating the amount of analyte in each of the standards and test samples.

Abstract: The present invention provides devices, kits and methods for the rapid multiplex quantitative analysis of analytes in a sample while eliminating the need for the end user to prepare standardized solutions of the analytes or internal standards. The devices of the present invention comprise multiwell plates manufactured to contain dried calibration standards, dried quality control standards, and dried internal standards and optionally contain tracers and deconjugation enzymes. The methods of the present invention do not require preparation and addition of these standards or optional components to a device, thus eliminating steps costly with regard to time and sample analyte measurement precision.