Abstract: Compositions and methods are provided for the efficient transformation of a dicot plant. More particularly, compositions and methods of the present invention find use in agriculture for Agrobacterium-mediated transformation of a dicotyledonous plant. The compositions include cultivation media comprising high concentrations of sucrose and glucose. The cultivation media find use in methods directed to Agrobacterium-mediated transformation of a dicot plant with a gene of interest. In this manner, any gene of interest can be introduced into a dicot plant with high transformation efficiency and reduced tissue necrosis.
April 3, 2012
Date of Patent:
February 24, 2015
Pioneer Bred International Inc, E.I. DuPont de Nemours
Hyeon-Je Cho, Linda A. Castle, Theodore M. Klein, Tai Minh, York Moy