Patents Assigned to Pioneer Hi-Bred International, Inc.
  • Publication number: 20210285006
    Abstract: Automated, high-throughput and multiplexed methods for clonal plant production from maternal or paternal derived cellular structures including embryos and microspores, using morphogenic factors to produce plants are disclosed. Large scale and rapid clonal propagation methods facilitate increased efficiency in producing doubled haploid plants for breeding and for trait introgression purposes.
    Type: Application
    Filed: March 9, 2021
    Publication date: September 16, 2021
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: WILLIAM JAMES GORDON-KAMM, JON AARON TUCKER REINDERS
  • Publication number: 20210277409
    Abstract: Spectinomycin resistant or streptomycin resistant transgenic plants and methods of making such plants are provided.
    Type: Application
    Filed: June 25, 2019
    Publication date: September 9, 2021
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: AJITH ANAND, HYEON-JE CHO, THEODORE M KLEIN
  • Publication number: 20210277070
    Abstract: The disclosure provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the disclosure provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
    Type: Application
    Filed: April 15, 2021
    Publication date: September 9, 2021
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: ANDRE ROGER ABAD, ANDREW CARL CROW, BRAD POLAND, JIMEI WANG
  • Publication number: 20210274739
    Abstract: Compositions and methods for generating maize plants that exhibit resistance to northern leaf blight are provided herein. Polynucleotides encoding a polypeptide that confers resistance to northern leaf blight, polynucleotide constructs comprising such, and maize plants comprising the polynucleotide constructs are provided.
    Type: Application
    Filed: May 13, 2021
    Publication date: September 9, 2021
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: ZHENGLIN HOU, APRIL L LEONARD, BAILIN LI, GIRMA M TABOR
  • Publication number: 20210254096
    Abstract: Compositions and methods are capable of modulating male fertility in a plant. Compositions comprise polynucleotides and polypeptides, and fragments and variants thereof, which modulate male fertility. Expression cassettes comprise a male-fertility polynucleotide, or fragment or variant thereof, operably linked to a promoter, wherein expression of the polynucleotide modulates the male fertility of a plant. The level and/or activity of a polynucleotide that influences male fertility is modulated in a plant or plant part. Regulatory sequences drive expression in a male-tissue-preferred manner and may be targets to downregulate an operably linked gene. Methods to track mutations that induce nuclear recessive male sterility in subsequent selfing and crossing of wheat lines containing the mutations are also provided. Male-sterile plants may be maintained by pollinating with a maintainer plant.
    Type: Application
    Filed: April 29, 2021
    Publication date: August 19, 2021
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: MARC C ALBERTSEN, UTE BAUMANN, ANDREW MARK CIGAN, MANJIT SINGH, ELISE TUCKER, RYAN WHITFORD
  • Publication number: 20210254095
    Abstract: Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.
    Type: Application
    Filed: April 22, 2021
    Publication date: August 19, 2021
    Applicants: PIONEER HI-BRED INTERNATIONAL, INC., E. I. DU PONT DE NEMOURS AND COMPANY
    Inventors: JENNIFER KARA BARRY, KEVIN HAYES, LU LIU, ERIC SCHEPERS, NASSER YALPANI
  • Patent number: 11090653
    Abstract: Microfluidic systems and methods to generate and analyze microcapsules comprising biological sample, such as for example, single cells, cellular contents, microspore, protoplast, are disclosed. The microcapsules comprising the biological sample can be preserved by a polymerization process that forms a hydrogel around the biological sample. The hydrogel microcapsules can be trapped in a trapping array or collected in an output reservoir and subject to one or more assays. The trapping array or the output reservoir can be disposed over a porous layer that can filter the continuous phase (e.g., oil) in which the microcapsules are dispersed in the microfluidic device. The pores of the porous layer are configured to be smaller than the size of the microcapsules to prevent the flow of the microcapsules through the porous layer.
    Type: Grant
    Filed: October 10, 2017
    Date of Patent: August 17, 2021
    Assignees: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: Abraham P. Lee, Dohyun Lee, Yue Yun
  • Publication number: 20210246465
    Abstract: Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.
    Type: Application
    Filed: April 28, 2021
    Publication date: August 12, 2021
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: DANIEL J ALTIER, JENNIFER KARA BARRY, RYAN MICHAEL GERBER, STEVEN D GRUVER, LU LIU, UTE SCHELLENBERGER, JUN-ZHI WEI, WEIPING XIE, NASSER YALPANI, GENHAI ZHU
  • Publication number: 20210238651
    Abstract: Methods for the high-throughput analysis of transgenic events are herein disclosed. The methods use libraries of sheared genomic DNA ligated to specialized adapters and pooled for sequence analysis and comparison to known genomic and insert sequence. The method finds use in detecting characterizing insertion site, transgene integrity, and transgene copy number.
    Type: Application
    Filed: February 8, 2021
    Publication date: August 5, 2021
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: MARY BEATTY, KEVIN HAYES, JENNA HOFFMAN, HAINING LIN, GINA MARIE ZASTROW-HAYES
  • Publication number: 20210238614
    Abstract: Methods and compositions are provided for the improvement of homology-directed repair of a double strand break in a plant cell, via the use of a polynucleotide comprising sequences homologous to the target site. In some aspects, the double strand break is created by an RNA-guided Cas endonuclease. The homology-directed repair of the double-strand break may include incorporation of a heterologous polynucleotide, for example a gene encoding a trait of agronomic importance. The homology-directed repair of the double-strand break may occur as a result of template-directed repair using a polynucleotide repair template.
    Type: Application
    Filed: May 7, 2019
    Publication date: August 5, 2021
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: AJITH ANAND, WILLIAM JAMES GORDON-KAMM, SANDEEP KUMAR, ZHAN-BIN LIU, SERGI SVITASHEV
  • Patent number: 11078546
    Abstract: Compositions and methods related to transgenic glyphosate tolerant Brassica plants are provided. Specifically, the present invention provides Brassica plants having a DP-073496-4 event which imparts tolerance to glyphosate. The Brassica plant harboring the DP-073496-4 event at the recited chromosomal location comprises genomic/transgene junctions within SEQ ID NO: 2 or with genomic/transgene junctions as set forth in SEQ ID NO: 12 and/or 13. The characterization of the genomic insertion site of the event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the event are provided.
    Type: Grant
    Filed: June 15, 2017
    Date of Patent: August 3, 2021
    Assignees: E. I. DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: David George Charne, Wenpin Chen, Chadwick Bruce Koscielny, Zhongsen Li, Jayantilal Devabhai Patel, Ferdinand Thoonen, Lomas Tulsieram, Yongping Zhang
  • Publication number: 20210230618
    Abstract: The present disclosure relates to the field of plant molecular biology, more particularly to regulation of gene expression in plants.
    Type: Application
    Filed: March 31, 2021
    Publication date: July 29, 2021
    Applicants: PIONEER HI-BRED INTERNATIONAL, INC., E. I. DU PONT DE NEMOURS AND COMPANY
    Inventors: ANDREW CARL CROW, SCOTT HENRY DIEHN, ALBERT LAURENCE LU, CARL ROBERT SIMMONS, LYNNE EILEEN SIMS
  • Publication number: 20210230622
    Abstract: The present disclosure relates to the field of plant molecular biology, more particularly to regulation of gene expression in plants.
    Type: Application
    Filed: May 20, 2019
    Publication date: July 29, 2021
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: ANDREW CARL CROW, SCOTT DIEHN, LYNNE EILEEN SIMS
  • Publication number: 20210222183
    Abstract: The disclosure provides plant, plant parts, plant cells, seed and grain containing a targeted genetic modification that inserts an inverted repeat sequence into a gene. The disclosure further provides hybrid plants that contain the inverted repeat sequence in a single allele of the gene. Further provided are methods for decreasing expression of a gene encoding a polypeptide of interest in a hybrid plant by crossing a plant comprising the targeted genetic modification with a plant not comprising the genetic modification to produce a hybrid plant that contains the inverted repeat sequence in a single allele of the gene.
    Type: Application
    Filed: January 19, 2021
    Publication date: July 22, 2021
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: HUIRONG GAO, JEFFREY HABBEN, SABRINA HUMBERT, JINRUI SHI
  • Publication number: 20210222192
    Abstract: Compositions and methods are provided for genome modification of a nucleotide sequence located in or near a male fertility gene of Ms1 or Ms5 in the genome of a plant cell or plant to produce a male-sterile plant. In some examples, the methods and compositions employ a guide RNA/Cas endonuclease system for modifying or altering target sites located in or near a male fertility gene of Ms1 or Ms5 in the genome of a plant cell, plant or seed to produce a male-sterile plant. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification a nucleotide sequence located in or near a male fertility gene of Ms1 or Ms5 in the genome of a plant cell to produce a male-sterile plant. Compositions and methods are also provided for restoring fertility to a Ms1 or Ms5 nucleotide sequence to a male-sterile Ms1 or Ms5 plant produced using the methods and compositions described herein.
    Type: Application
    Filed: December 10, 2018
    Publication date: July 22, 2021
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: UTE BAUMANN, ANDREW MARK CIGAN, MARGARET ANNE PALLOTTA, MANJIT SINGH, RADOSLAW SUCHECKI, NATHAN SPENCER WATSON-HAIGH, RYAN WHITFORD
  • Patent number: 11059043
    Abstract: A microfluidic system includes a microfluidic chip including a channel layer and a fluid control layer operatively connected to the channel layer, the channel layer having one or more fluid channels. The one or more channels are configured to contain a plurality of droplets. A valve control system is provided to control flow of fluid through the one or more fluid channels in the channel layer. The microfluidic system also includes a droplet impedance detection and feedback control system operatively connected to the valve control system. The droplet impedance detection and feedback control system is configured to detect at least a position of at least one droplet in a fluid channel and to send a signal to the valve control system to operate a particular valve at a particular time based on the detected position of the at least one droplet.
    Type: Grant
    Filed: April 19, 2017
    Date of Patent: July 13, 2021
    Assignees: The Johns Hopkins University, Pioneer Hi-Bred International, Inc.
    Inventors: Tza-Huei Wang, Brant Axt, Yi-Fan Hsieh, Helena C. Zec, Aniruddha Kaushik, Kuangwen Hsieh, Yue Yun
  • Publication number: 20210207225
    Abstract: Various methods and compositions are provided for identifying and/or selecting a sorghum plant or germplasm with or without a cytoplasmic male sterility (CMS) trait. In certain embodiments, the method comprises detecting at least one allele of one or more marker locus within or linked to a QTL associated with CMS. In further embodiments, the method comprises crossing a selected sorghum plant with a recurrent sorghum parent plant and selecting progeny with CMS.
    Type: Application
    Filed: May 30, 2019
    Publication date: July 8, 2021
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: SUNITA R CHILAKAMARRI, DAVID ALAN HESSEL, SIVA S AMMIRAJU JETTY, MARIA LAURA MAYOR
  • Publication number: 20210210163
    Abstract: Systems and methods for improving marker-trait associations and for improving trait introgression precision while reducing trait introgression time are disclosed. Genes responsible for recombination are edited to reduce function, and thus increase recombination rates. Increased recombination rates allow more precise quantification of marker-trait associations, and more precise and faster trait introgression. Methods and compositions useful for selecting an organism with a trait of interest are provided herein. Candidate organisms identified and/or selected by any of the methods described above are also of interest.
    Type: Application
    Filed: May 24, 2019
    Publication date: July 8, 2021
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: ANDREW BAUMGARTEN, JIN LI, GREGORY D MAY
  • Publication number: 20210207153
    Abstract: Systems and methods for editing plant genomes include systems and methods for transforming plants generally, and Brassica plants particularly, with genome editing systems including CRISPR-Cas. Systems and methods for editing plant genomes include systems and methods for transforming Brassica microspores with genome editing systems and culturing the plant microspores that increase genome editing frequency.
    Type: Application
    Filed: May 30, 2019
    Publication date: July 8, 2021
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: David George Charne, Wenpin Chen, James Brent Gillespie, Siva S. Ammirajo Jetty, Zhan-Bin Liu, Gina Marie Zastrow-Hayes
  • Publication number: 20210207103
    Abstract: Provided are compositions comprising polynucleotides encoding modified diacylglycerol acyltransferase-1 (DGAT1) polypeptides having improved properties, such as increased enzymatic activity and/or increased stability. Plants, plant cells, seed, grain and comprising the polynucleotides are provided which have one or more of increased fatty acid or protein content. Methods of generating the polynucleotides in plant cells include transformation and genetic modification. Methods of employing the polynucleotides in plants, methods for increasing DGAT1 activity in a plant, and methods for increasing fatty acid content or protein content in a plant are provided.
    Type: Application
    Filed: May 30, 2019
    Publication date: July 8, 2021
    Applicant: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: KAYLA S FLYCKT, KRISTIN HAUG COLLET, ZHAN-BIN LIU, KEITH R ROESLER, BO SHEN, LAURA L WAYNE