Abstract: A biocatalytic process for producing active pharmaceutical ingredients (APIs) or intermediates thereof, wherein those APIs or their intermediates are nucleoside analogues (NAs) of formula I and wherein said NAs are active as pharmaceutically relevant antivirals and anticancer medicaments, intermediates or prodrugs thereof.
Type:
Grant
Filed:
April 29, 2014
Date of Patent:
January 22, 2019
Assignee:
Plasmia Biotech, S.L.
Inventors:
Marta Pascual Gilabert, Victor Manuel Deroncelé Thomas, Rafael Montilla Arévalo
Abstract: A recombinant expression vector comprising: a) the sequence encoding a purine nucleoside phosphorylase (PNPase, E. C. 2.4.2.1), b) the sequence encoding a uridine phosphorylase (UPase, E. C. 2.4.2.3), c) or both; each of the sequences operably linked to one or more control sequences that direct the production of said phosphorylases in a suitable expression host; said sequences originating from the Archaea Thermoprotei class, characterized in that the PNPase is from Sulfolobus solfataricus (SEQ ID NO. 7) and the UPase is from Aeropyrum pernix (SEQ ID NO. 8). In addition, the present invention relates to A transglycosylation method between a sugar-donating nucleoside and an acceptor base in the presence of phosphate ions, characterized in that said method comprises the use of a uridine phosphorylase (UPase) of Aeropyrum pernix (NC_000854.2), a purine nucleoside phosphorylase (PNpase) of Sulfolobus solfataricus (NC_002754.1), or a combination thereof.
Type:
Grant
Filed:
July 22, 2013
Date of Patent:
June 24, 2014
Assignee:
Plasmia Biotech, S.L.
Inventors:
Rafael Montilla Arevalo, Victor Manuel DeronceléThomas, Cristina López Gómez, Marta Pascual Gilabert, Carlos Estevez Company, Josep Castells Boliart
Abstract: The present invention relates to a recombinant expression vector comprising: a) the sequence encoding a purine nucleoside phosphorylase (PNPase, E. C. 2.4.2.1), b) the sequence encoding a uridine phosphorylase (UPase, E. C. 2.4.2.3), c) or both; each of the sequences operably linked to one or more control sequences that direct the production of said phosphorylases in a suitable expression host; said sequences originating from the Archaea Thermoprotei class, characterized in that the PNPase is from Sulfolobus solfataricus (SEQ ID NO. 7) and the UPase is from Aeropyrum pernix (SEQ ID NO. 8). In addition, the present invention relates to A transglycosylation method between a sugar-donating nucleoside and an acceptor base in the presence of phosphate ions, characterised in that said method comprises the use of a uridine phosphorylase (UPase) of Aeropyrum pernix (NC_000854.2), a purine nucleoside phosphorylase (PN-Pase) of Sulfolobus solfataricus (NC_002754.1), or a combination thereof.
Type:
Grant
Filed:
December 22, 2010
Date of Patent:
August 20, 2013
Assignee:
Plasmia Biotech, S.L.
Inventors:
Rafael Montilla Arevalo, Víctor Manuel Deroncelé Thomas, Cristina López Gómez, Marta Pascual Gilabert, Carlos Estévez Company, Josep Castells Boliart