Abstract: The present invention relates to processes for the isolation of a phospholipid and for producing a polyunsaturated, long-chain fatty acids (PUFA)-enriched fraction from a fish oil comprising the steps of -providing a fish oil containing lipids and phospholipids; -mixing the fish oil with a polar solvent; -centrifuging the mixture of the fish oil and the polar solvent to separate a polar fraction from a lipid fraction; -isolating a phospholipid from the polar fraction or isolating a PUFA-enriched fraction from the polar fraction. The fish oil may be provided by -extracting a fish material with an extractant solvent; -removing the extractant solvent to provide the fish oil; -optionally subjecting the fish oil to a solid-liquid separation. The isolated phospholipids and PUFA's may be used as additives for functional foods, as a dietary supplement and for pharmaceutical application.