Abstract: The present invention provides a polypeptide as standard for peptide analysis by mass spectrometry comprising at least 16 peptides selected from the group consisting of the peptides of SEQ ID NO: 1 to SEQ ID NO: 22 or variants thereof, together with an artificial protein comprising the polypeptide, a vector comprising a nucleic acid encoding the polypeptide, a kit for proteome analysis, a selection of peptides for calibration an devaluation of mass spectrometers and chromatographs for proteome analysis and uses thereof.

Abstract: The invention provides an artificial protein for quantitative analysis of the proteome of a sample, cell or organism, comprising at least two consecutive peptides linked by a cleavage sequence for separating the peptides; a singular marker on one or more peptide for determination of the absolute amount of this fragment; and N-terminal and C-terminal extensions for protection of the peptides; wherein each peptide represents one single protein of the sample, cell or organism and each peptide is in a defined stoichiometry. The invention further provides a collection of peptides, a vector and a kit comprising the artificial protein and a method for quantitative analysis of the proteome.