Abstract: Compositions, methods, and applications for single component systems for the formation of in situ hydrogels are described. The single component systems are effective for forming stable, single solutions containing precursors providing both electrophilic and nucleophilic groups that chemically crosslink on contact with physiological fluids associated with vital physiological tissue. The single component systems can be applied using various delivery vehicles, including injections, needle and needless catheters, and sprays. The single component systems can be useful for enabling multiple injections from a single syringe through a small gauge needle or other dispenser. Methods for transcervical installation of in situ formed hydrogels into one or more fallopian tubes are described.