Abstract: Disclosed herein are methods of detecting sepsis in a patient in need thereof, the method comprising: receiving a patient sample, a pathogenic primer mix, a fluorophore label probe, and a dPCR buffer in one or more wells of a microfluidic device and detecting fluorescent light signal from the droplets to determine sepsis in the patient. The microfluidic device comprises a droplet generation channel in fluid communication with the one or more wells, adapted to generate droplets comprising the patient sample, one or more pathogenic primers, a fluorophore label probe, and dPCR buffer. Furthermore, the microfluidic device comprises a chamber in fluid communication with the droplet generation channel for collecting the droplets generated by the droplet generation channel and performing a PCR reaction in the droplets.

Abstract: Disclosed herein are pressure control systems, comprising: a touch screen electrically connected to industrial computer or embedded operating system; a pressure output channel connected to the touch screen and/or the industrial computer or embedded operating system; and pressure control unit for communicating with the pressure output channel, wherein the pressure control unit can be controlled with the touch screen, and wherein the industrial computer or embedded operating system comprises preinstalled software with User Interface (UI) for pressure control setup and running the system.

Abstract: Disclosed herein are microfluidic devices and systems for amplifying and detecting a target polynucleotide, comprising: one or more wells for receiving one or more substrates; a droplet generation channel in fluid communication with the one or more wells, wherein the microfluidic channel is adapted to generate droplets; and a chamber in fluid communication with the droplet generation channel, and adapted to collect droplets generated by the droplet generation channel, and further adapted to perform nucleic acid amplification in droplets, and further adapted to detect light signal from droplets. Also disclosed are methods of using the same.

Abstract: Disclosed herein are systems comprising a pressure adjustment manifold, a pressure distribution manifold, and an electronic board, each of which are operatively connected. Also provided herein are methods of making and using the same.

Abstract: Disclosed herein are microfluidic devices and systems for amplifying and detecting a target polynucleotide, comprising: one or more wells for receiving one or more substrates; a droplet generation channel in fluid communication with the one or more wells, wherein the microfluidic channel is adapted to generate droplets; and a chamber in fluid communication with the droplet generation channel, and adapted to collect droplets generated by the droplet generation channel, and further adapted to perform nucleic acid amplification in droplets, and further adapted to detect light signal from droplets. Also disclosed are methods of using the same.

Abstract: Disclosed herein are microfluidic devices comprising, one or more inlets in flow communication with one or more microfluidic channels, wherein the one or more inlets are adapted for receiving deformable beads, oil, and/or a suspension comprising buffer, cells, and/or particles, wherein the one or more microfluidic channels are in flow communication with the one or more inlets through a cross junction and define a fluid flow path therebetween, said fluid flow path forming a substantially planar substrate, and wherein the microfluidic channel is adapted to generate droplets. Also disclosed are methods of making and using the same.