Abstract: The present invention is related to a method to be performed with one tissue type, wherein a specific combination of hydrogel features has been pre-selected for the said one tissue type to be tested. The present invention is also related to a kit of parts to perform said method.
Type:
Application
Filed:
December 1, 2020
Publication date:
February 9, 2023
Applicant:
PRECISION CANCER TECHNOLOGIES INC .
Inventors:
Simone RIZZI, Jeremy TOUATI, Giulia FREGNI, Cara BUCHANAN PISANO, Franck COUMAILLEAU, Emanuele GAUDIELLO, Sophie GRETTAZ, Nicolas CHARTIER, Mathieu HEULOT
Abstract: The present invention is related to a biofunctional three-dimensional hydrogel suitable for the expansion of freshly isolated or frozen intestinal cells and the formation of intestinal organoids therefrom, that avoids the use of a naturally-derived matrix such as Matrigel and provides intestinal organoids suitable for clinical applications and generated in a commercially feasible manner. The present invention is also related to a kit of parts comprising said hydrogel in combination with a suitable culture medium, and to a method of making organoids and expanding cells from freshly isolated or frozen intestinal cells with said kit of parts.
Type:
Application
Filed:
August 24, 2020
Publication date:
September 22, 2022
Applicant:
PRECISION CANCER TECHNOLOGIES INC.
Inventors:
Simone RIZZI, Jeremy TOUATI, Giulia FREGNI, Cara BUCHANAN PISANO, Franck COUMAILLEAU, Mathieu HEULOT