Patents Assigned to President and Fellows of Harvard College
  • Publication number: 20220195418
    Abstract: The invention provides systems, methods, reagents, apparatuses, vectors, and host cells for the continuous evolution of nucleic acids. For example, a lagoon is provided in which a population of viral vectors comprising a gene of interest replicates in a stream of host cells, wherein the viral vectors lack a gene encoding a protein required for the generation of infectious viral particles, and wherein that gene is expressed in the host cells under the control of a conditional promoter, the activity of which depends on a function of the gene of interest to be evolved. Some aspects of this invention provide evolved products obtained from continuous evolution procedures described herein. Kits containing materials for continuous evolution are also provided.
    Type: Application
    Filed: December 3, 2021
    Publication date: June 23, 2022
    Applicant: President and Fellows of Harvard College
    Inventors: David R. Liu, Kevin Michael Esvelt, Jacob Charles Carlson
  • Publication number: 20220195503
    Abstract: The invention provides for systems, methods, and compositions for targeting nucleic acids. In particular, the invention provides non-naturally occurring or engineered DNA-targeting systems comprising a novel DNA-targeting CRISPR effector protein and at least one targeting nucleic acid component like a guide RNA. Methods for making and using and uses of such systems, methods, and compositions and products from such methods and uses are also disclosed and claimed.
    Type: Application
    Filed: December 17, 2021
    Publication date: June 23, 2022
    Applicants: The Broad Institute, Inc., Massachusetts Institute of Technology, President and Fellows of Harvard College
    Inventors: Feng ZHANG, Bernd ZETSCHE, Johnathan S. GOOTENBERG, Omar O. ABUDAYYEH, Ian SLAYMAKER
  • Publication number: 20220195457
    Abstract: Provided are therapeutic virus vectors, particularly, recombinant adeno-associated virus (rAAV) vectors, designed to contain an enhancer sequence that specifically restricts expression of an effector gene (e.g., an SCN1A-encoding polynucleotide, Gq-DREADD-encoding polynucleotide, or PSAM-encoding polynucleotide) contained in the vector to PV-expressing GABAergic interneuron or to neuron cell populations in the brain. The rAAV vectors, compositions and methods thereof are useful for treating subjects afflicted with neuropathologies, seizures, pharmacologically-intractable forms of epilepsy including Dravet syndrome (DS), a form of infantile epilepsy associated with severe seizures, cognitive impairment and premature death, as the cause of DS involves loss of function of a sodium channel encoded by the SCN1A gene.
    Type: Application
    Filed: January 27, 2020
    Publication date: June 23, 2022
    Applicants: THE BROAD INSTITUTE, INC., PRESIDENT AND FELLOWS OF HARVARD COLLEGE, NEW YORK UNIVERSITY
    Inventors: Jordane DIMIDSCHSTEIN, Gordon FISHELL, Orrin DEVINSKY
  • Publication number: 20220195017
    Abstract: Described herein are engineered microbe-targeting or microbe-binding molecules, kits comprising the same and uses thereof. The microbe-targeting or microbe-binding molecules can comprise a microbe surface-binding domain linked to a portion of an Fc region. Further, the microbe-targeting molecules can be conjugated to substrate (e.g., a magnetic particle) to form a microbe-targeting substrate. Such microbe-targeting molecules and/or substrates and the kits comprising the same can be used in various applications, such as diagnosis and/or treatment of an infection caused by microbes. Moreover, the microbe-targeting molecules and/or substrates can be easily regenerated after use.
    Type: Application
    Filed: December 30, 2021
    Publication date: June 23, 2022
    Applicant: President and Fellows of Harvard College
    Inventors: Donald E. Ingber, Michael Super, Alexander L. Watters
  • Publication number: 20220192986
    Abstract: The invention provides compositions and methods to form pores in situ within hydrogels following hydrogel injection. Pores formed in situ via degradation of sacrificial porogens within the surrounding hydrogel facilitate recruitment or release of cells. Disclosed herein is a material that is not initially porous, but which becomes macroporous over time.
    Type: Application
    Filed: November 9, 2021
    Publication date: June 23, 2022
    Applicant: President and Fellows of Harvard College
    Inventors: Nathaniel D. Huebsch, Christopher M. Madl, Kangwon Lee, Maria M. Xu, David J. Mooney
  • Publication number: 20220193021
    Abstract: Provided herein, in some aspects, are combination therapies for inhibiting influenza virus infection.
    Type: Application
    Filed: April 30, 2020
    Publication date: June 23, 2022
    Applicant: President and Fellows of Harvard College
    Inventors: Longlong Si, Rachelle Prantil-Baun, Kambez Hajipouran Benam, Melissa Rodas, Ratnakar Potla, Donald E. Ingber
  • Publication number: 20220193034
    Abstract: Provided herein are compounds useful for the treatment of various parasitic diseases. These compounds, as well as pharmaceutically acceptable salts thereof may be formulated in pharmaceutical compositions, veterinary compositions and may be used in methods of treatment and/or prophylaxis of diseases spread by parasites, including cryptosporidiosis.
    Type: Application
    Filed: April 22, 2020
    Publication date: June 23, 2022
    Applicants: WASHINGTON UNIVERSITY, THE BROAD INSTITUTE, INC., PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Laurence David Sibley, Bruno Melillo, Eamon Comer
  • Patent number: 11365429
    Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.
    Type: Grant
    Filed: April 29, 2019
    Date of Patent: June 21, 2022
    Assignee: President and Fellows of Harvard College
    Inventors: George M. Church, Prashant G. Mali, Luhan Yang
  • Patent number: 11365410
    Abstract: Methods of making and monitoring metabolites in cells are provided.
    Type: Grant
    Filed: April 12, 2016
    Date of Patent: June 21, 2022
    Assignee: President and Fellows of Harvard College
    Inventors: Jameson K. Rogers, George M. Church
  • Patent number: 11366100
    Abstract: The present invention is based, in part, on the identification of novel FI.3K-mTORCI-S6K 1 signaling pathway biomarkers predictive of responsiveness to anti-cancer therapies.
    Type: Grant
    Filed: February 12, 2015
    Date of Patent: June 21, 2022
    Assignees: Dana-Farber Cancer Institute, Inc., President and Fellows of Harvard College
    Inventors: Thomas M. Roberts, Haoxuan Tong, Jean Zhao, John Blenis
  • Patent number: 11366296
    Abstract: A method of fabricating a visible spectrum optical component includes: providing a substrate; forming a resist layer over a surface of the substrate; patterning the resist layer to form a patterned resist layer defining openings exposing portions of the surface of the substrate; performing deposition to form a dielectric film over the patterned resist layer and over the exposed portions of the surface of the substrate, wherein a top surface of the dielectric film is above a top surface of the patterned resist layer; removing a top portion of the dielectric film to expose the top surface of the patterned resist layer and top surfaces of dielectric units within the openings of the patterned resist layer; and removing the patterned resist layer to retain the dielectric units over the substrate.
    Type: Grant
    Filed: November 23, 2016
    Date of Patent: June 21, 2022
    Assignees: PRESIDENT AND FELLOWS OF HARVARD COLLEGE, THE CHARLES STARK DRAPER LABORATORY, INC.
    Inventors: Robert C. Devlin, Mohammadreza Khorasaninejad, Federico Capasso, Hongkun Park, Alexander Arthur High
  • Publication number: 20220186301
    Abstract: The technology described herein is directed to methods, systems, and compositions for analyzing, detecting, and/or visualizing target molecules. Described herein are compositions and systems comprising oligonucleotide tags comprising barcoded cassettes. Also described herein are methods for analyzing target molecules, the method comprising contacting a sample with at least one oligonucleotide tag, contacting the sample with at least two readout molecules, and detecting the relative spatial order of the readout molecules.
    Type: Application
    Filed: July 30, 2020
    Publication date: June 16, 2022
    Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Huy Quoc NGUYEN, Shyamtanu CHATTORAJ, George M. CHURCH, Chao-ting WU
  • Patent number: 11358105
    Abstract: The present invention generally relates to systems and methods for the control of fluids and, in some cases, to systems and methods for flowing a fluid into and/or out of other fluids. As examples, fluid may be injected into a droplet contained within a fluidic channel, or a fluid may be injected into a fluidic channel to create a droplet. In some embodiments, electrodes may be used to apply an electric field to one or more fluidic channels, e.g., proximate an intersection of at least two fluidic channels. For instance, a first fluid may be urged into and/or out of a second fluid, facilitated by the electric field. The electric field, in some cases, may disrupt an interface between a first fluid and at least one other fluid. Properties such as the volume, flow rate, etc.
    Type: Grant
    Filed: January 11, 2021
    Date of Patent: June 14, 2022
    Assignee: President and Fellows of Harvard College
    Inventors: David A. Weitz, Adam R. Abate, Tony Hung, Pascaline Mary
  • Patent number: 11359020
    Abstract: The present invention relates to compositions and methods for the immunomodulation mediated by the interaction of PD-L2 and RGMb.
    Type: Grant
    Filed: September 16, 2019
    Date of Patent: June 14, 2022
    Assignees: Dana-Farber Cancer Institute, Inc., President and Fellows of Harvard College, Children's Medical Center Corporation
    Inventors: Gordon J. Freeman, Arlene H. Sharpe, Yanping Xiao, Loise Francisco, Rosemarie Dekruyff, Dale Umetsu
  • Patent number: 11360174
    Abstract: Systems and methods are disclosed for a pulse sequence that reduces disorder and/or interaction effects in spin systems. A protocol can be used to design a pulse sequence that includes altering the frame orientation of the spin system with each electromagnetic pulse in the pulse sequence. The frame orientations during the sequence can conform to certain conditions. The number positive rotations along each axis can be the same as the number negative rotations along the respective axis. The number of rotations along one axis should be the same as the number of rotations along the other axes.
    Type: Grant
    Filed: March 6, 2020
    Date of Patent: June 14, 2022
    Assignee: President and Fellows of Harvard College
    Inventors: Mikhail D. Lukin, Hengyun Zhou, Joonhee Choi, Soonwon Choi, Helena Knowles, Renate Landig
  • Patent number: 11359199
    Abstract: Described herein are methods and compositions related to the modulation of progranulin expression or activity in the brain for the treatment of neurodegenerative diseases.
    Type: Grant
    Filed: August 25, 2021
    Date of Patent: June 14, 2022
    Assignees: PRESIDENT AND FELLOWS OF HARVARD COLLEGE, THE CHILDREN'S MEDICAL CENTER CORPORATION
    Inventors: Peter Jungsoo Park, Jin Kuk Kim, Timothy Yu, Yu-Han Huang
  • Patent number: 11359229
    Abstract: Provided herein are molecular verification (authentication) systems, methods and compositions that use molecular (DNA/RNA) circuitry to enable specific molecularly encrypted solutions.
    Type: Grant
    Filed: September 19, 2017
    Date of Patent: June 14, 2022
    Assignee: President and Fellows of Harvard College
    Inventors: Jongmin Kim, Jocelyn Yoshiko Kishi, Peng Yin, Steven Henry Strassmann, Thomas E. Schaus
  • Patent number: 11359211
    Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.
    Type: Grant
    Filed: April 29, 2019
    Date of Patent: June 14, 2022
    Assignee: President and Fellows of Harvard College
    Inventors: George M. Church, Prashant G. Mali, Luhan Yang
  • Patent number: 11361845
    Abstract: Methods and systems for designing, testing, and validating genome designs based on rules or constraints or conditions or parameters or features and scoring are described herein.
    Type: Grant
    Filed: June 15, 2017
    Date of Patent: June 14, 2022
    Assignee: President and Fellows of Harvard College
    Inventors: Gleb Kuznetsov, Marc J. Lajoie, Matthieu M. Landon, Michael G. Napolitano, Daniel Bryan Goodman, Christopher J. Gregg, George M. Church, Nili Ostrov
  • Patent number: 11359013
    Abstract: The present invention provides methods and compositions for the treatment, prevention, or reduction of persistent infections, such as chronic infections, latent infections, and slow infections and cancer. The methods and compositions of the invention are also useful for the alleviation of one or more symptoms associated with such infections and cancer.
    Type: Grant
    Filed: June 18, 2019
    Date of Patent: June 14, 2022
    Assignees: Emory University, Dana-Farber Cancer Institute, Inc., Brigham and Women's Hospital, Inc., President and Fellows of Harvard College
    Inventors: Gordon Freeman, Arlene Sharpe, David M. Dorfman, Rafi Ahmed, Daniel Barber, E. John Wherry