Patents Assigned to President & Fellows of Harvard College
  • Barcoding systems and methods for gene sequencing and other applications
    Patent number: 11746367
    Abstract: The present invention generally relates to microfluidics and labeled nucleic acids. In one aspect, the present invention is generally directed to a method, wherein the method includes providing a plurality of droplets comprising particles, the particles comprising oligonucleotides, and attaching a nucleic acid sequence to the oligonucleotides. Certain embodiments are generally directed to systems and methods for splitting a droplet into two or more droplets. Certain embodiments are generally directed to systems and methods for sorting fluidic droplets in a liquid.
    Type: Grant
    Filed: April 15, 2016
    Date of Patent: September 5, 2023
    Assignee: President and Fellows of Harvard College
    Inventors: David A. Weitz, Huidan Zhang, John Heyman, Allon Moshe Klein
  • DNA-guided gene editing and regulation
    Patent number: 11746349
    Abstract: Methods and compositions of altering a eukaryotic cell are described including providing to the eukaryotic cell a guide DNA sequence complementary to a target nucleic acid sequence, providing to the eukaryotic cell an Ago enzyme or a nuclease null Ago protein that interacts with the guide DNA sequence for DNA-guided gene editing and regulation of the target nucleic acid sequence in a site specific manner.
    Type: Grant
    Filed: February 7, 2017
    Date of Patent: September 5, 2023
    Assignee: President and Fellows of Harvard College
    Inventors: George M. Church, Luhan Yang, Margo R. Monroe, Po-Yi Huang
  • Apparatuses for cell mapping via impedance measurements and methods to operate the same
    Patent number: 11747321
    Abstract: Disclosed herein are an apparatus for electrically assessing and/or manipulating cells. One aspect is directed to electrically mapping cells on the surface of the semiconductor substrate via cross-electrode impedance measurements. Further according to some aspects, the electrode array allows for spatially addressable electrical stimulation and/or recording of electrical signals in real-time using the CMOS circuitry. Some of these aspects are directed to using an electrode array to perform cell patterning through electrochemical gas generation, and extracellular electrochemical mapping.
    Type: Grant
    Filed: July 29, 2022
    Date of Patent: September 5, 2023
    Assignee: President and Fellows of Harvard College
    Inventors: Donhee Ham, Jeffrey T. Abbott, Wenxuan Wu, Tianyang Ye, Han Sae Jung, Hongkun Park
  • METHODS AND COMPOSITIONS FOR EVOLVING BASE EDITORS USING PHAGE-ASSISTED CONTINUOUS EVOLUTION (PACE)
    Publication number: 20230272425
    Abstract: The instant specification provides for evolved base editors which overcome deficiencies of those in art (including increased efficiency and/or decreased requirement for specific sequence-context at an editing site) and which are obtained a result of a phage-assisted continuous evolution (PACE) system. In particular, the instant specification provides for evolved cytidine base editors (e.g., based on APOBEC1, CDA, or AID cytidine deaminase domains) which overcome deficiencies of those in art (including increased efficiency and/or decreased requirement for specific sequence-context at an editing site) and which are obtained a result of a phage-assisted continuous evolution (PACE) system.
    Type: Application
    Filed: March 3, 2023
    Publication date: August 31, 2023
    Applicants: President and Fellows of Harvard College, The Broad Institute, Inc.
    Inventors: David R. Liu, Benjamin Thuronyi, Christopher Gerard Wilson
  • MULTIPLEXED METHODS FOR DETECTING TARGET RNAS
    Publication number: 20230265484
    Abstract: The technology described herein is directed to methods, kits, compositions, and systems for detecting a target RNA, such as a small amount of viral RNA. In one aspect, described herein are methods of detecting the target RNA, using primers comprising at least one barcode region. In other aspects, described herein are kits, compositions, and systems suitable to practice the methods described herein to detect the target RNA.
    Type: Application
    Filed: March 24, 2021
    Publication date: August 24, 2023
    Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Marc W. KIRSCHNER, Mingjie DAI, George M. CHURCH
  • Engineered guide RNA sequences for in situ detection and sequencing
    Patent number: 11732258
    Abstract: A functional engineered guide RNA sequence is provided including a spacer sequence and a scaffold sequence, wherein the scaffold sequence includes a primer binding site for reverse transcription.
    Type: Grant
    Filed: November 1, 2017
    Date of Patent: August 22, 2023
    Assignee: President and Fellows of Harvard College
    Inventors: Reza Kalhor, Prashant G. Mali, George M. Church
  • Achromatic metasurface optical components by dispersive phase compensation
    Patent number: 11733535
    Abstract: Multi-wavelength light is directed to an optic including a substrate and achromatic metasurface optical components deposited on a surface of the substrate. The achromatic metasurface optical components comprise a pattern of dielectric resonators. The dielectric resonators have nonperiodic gap distances between adjacent dielectric resonators; and each dielectric resonator has a width, w, that is distinct from the width of other dielectric resonators. A plurality of wavelengths of interest selected from the wavelengths of the multi-wavelength light are deflected with the achromatic metasurface optical components at a shared angle or to or from a focal point at a shared focal length.
    Type: Grant
    Filed: September 23, 2020
    Date of Patent: August 22, 2023
    Assignee: President and Fellows of Harvard College
    Inventors: Francesco Aieta, Mikhail Kats, Patrice Genevet, Federico Capasso, Mohammadreza Khorasaninejad
  • Methods and compositions for evolving base editors using phage-assisted continuous evolution (PACE)
    Patent number: 11732274
    Abstract: The instant specification provides for evolved base editors which overcome deficiencies of those in art (including increased efficiency and/or decreased requirement for specific sequence-context at an editing site) and which are obtained a result of a phage-assisted continuous evolution (PACE) system. In particular, the instant specification provides for evolved cytidine base editors (e.g., based on APOBEC1, CDA, or AID cytidine deaminase domains) which overcome deficiencies of those in art (including increased efficiency and/or decreased requirement for specific sequence-context at an editing site) and which are obtained a result of a phage-assisted continuous evolution (PACE) system.
    Type: Grant
    Filed: July 27, 2018
    Date of Patent: August 22, 2023
    Assignees: President and Fellows of Harvard College, The Broad Institute, Inc.
    Inventors: David R. Liu, Ben Thuronyi, Christopher Gerard Wilson
  • Delivery of therapeutic RNAs via ARRDC1-mediated microvesicles
    Patent number: 11730823
    Abstract: Methods, systems, compositions and strategies for the delivery of RNA into cells in vivo, ex vivo, or in vitro via ARMMs are provided. In some aspects, ARMMs containing fusion proteins of ARRDC1 fused to an RNA binding protein or an RNA binding protein fused to a WW domain are provided. In some aspects, ARMMs containing binding RNAs associated with cargo RNAs are provided. In other aspects, cargo RNAs associated with a binding RNA, such as a TAR element, are loaded into ARMMs via ARRDC1 fusion proteins containing an RNA binding protein, such as trans-activator of transcription (Tat) protein.
    Type: Grant
    Filed: October 3, 2017
    Date of Patent: August 22, 2023
    Assignee: President and Fellows of Harvard College
    Inventor: Quan Lu
  • CASCADED-MODE RESONATORS
    Publication number: 20230258867
    Abstract: A device includes a first mode converter and a second mode converter that define a region between the first mode converter and the second mode converter. The region can contain a plurality of orthogonal modes of a wave. The wave, when sent from outside the region and when propagating from the first mode converter towards the second mode converter, can include a first mode of the plurality of orthogonal modes. The second mode converter can convert the wave from the first mode of the plurality of orthogonal modes, to a second mode of the plurality of orthogonal modes that is different from the first mode. The first mode converter can convert the wave to the first mode of the plurality of orthogonal modes.
    Type: Application
    Filed: February 15, 2023
    Publication date: August 17, 2023
    Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Vincent Ginis, Ileana-Cristina BENEA-CHELMUS, Jinsheng LU, Marco PICCARDO, Federico CAPASSO
  • ARTIFICIAL SECRETION PEPTIDES FOR HETEROLOGOUS PROTEIN PRODUCTION
    Publication number: 20230257794
    Abstract: Provided herein, in some embodiments, are artificial secretion peptides capable of directing secretion from Lactobacillus for use, for example, in producing heterologous proteins, including therapeutic proteins.
    Type: Application
    Filed: November 14, 2022
    Publication date: August 17, 2023
    Applicant: President and Fellows of Harvard College
    Inventors: Anik Debnath, George M. Church
  • Cleavable nucleotide analogs and uses thereof
    Patent number: 11725222
    Abstract: Cleavable nucleotide analogs are provided. The nucleotide analog includes a nucleotide molecule attached to a cleavable moiety wherein the cleavable moiety comprises a protective group and/or a linker attached to a fluorophore. The cleavable moiety is linked to the oxygen atom of the 3?-OH of the pentose of the nucleotide molecule. The nucleotide analogs can be used in making polynucleotide molecules using template independent polymerases. The nucleotide analogs can act as reversible terminators during DNA sequencing by synthesis. The cleavage of the cleavable moiety restores a free 3?-OH functional group allowing growth of the polynucleotide molecule. The general structures as well as proposed synthetic schemes for the nucleotide analogs are also provided.
    Type: Grant
    Filed: November 30, 2017
    Date of Patent: August 15, 2023
    Assignee: President and Fellows of Harvard College
    Inventors: Kettner John Frederick Griswold, Jr., Richard E. Kohman, George M. Church, Jonathan Rittichier
  • Fluid injection
    Patent number: 11724237
    Abstract: The present invention generally relates to systems and methods for the control of fluids and, in some cases, to systems and methods for flowing a fluid into and/or out of other fluids. As examples, fluid may be injected into a droplet contained within a fluidic channel, or a fluid may be injected into a fluidic channel to create a droplet. In some embodiments, electrodes may be used to apply an electric field to one or more fluidic channels, e.g., proximate an intersection of at least two fluidic channels. For instance, a first fluid may be urged into and/or out of a second fluid, facilitated by the electric field. The electric field, in some cases, may disrupt an interface between a first fluid and at least one other fluid. Properties such as the volume, flow rate, etc.
    Type: Grant
    Filed: May 16, 2022
    Date of Patent: August 15, 2023
    Assignee: President and Fellows of Harvard College
    Inventors: David A. Weitz, Adam R. Abate, Tony Hung, Pascaline Mary
  • Quinones having high capacity retention for use as electrolytes in aqueous redox flow batteries
    Patent number: 11724980
    Abstract: We disclose quinone compounds and related species (Formula I) that possess significant advantages when used as a redox active material in a battery, e.g., a redox flow battery. In particular, the compounds provide redox flow batteries (RFBs) with extremely high capacity retention. For example, RFBs of the invention can be cycled for 500 times with negligible loss of capacity, and such batteries could be employed for years of service. Thus, the invention provides a high efficiency, long cycle life redox flow battery with reasonable power cost, low energy cost, and all the energy scaling advantages of a flow battery.
    Type: Grant
    Filed: February 11, 2019
    Date of Patent: August 15, 2023
    Assignee: President and Fellows of Harvard College
    Inventors: Michael J. Aziz, Roy G. Gordon, Kaixiang Lin, David Gator Kwabi, Yunlong Ji
  • GDF11 variants and uses thereof
    Patent number: 11725033
    Abstract: Disclosed herein are GDF11 variant polypeptides. Also disclosed herein are methods for increasing GDF11 protein levels in a subject by administering a GDF11 variant polypeptide.
    Type: Grant
    Filed: July 20, 2020
    Date of Patent: August 15, 2023
    Assignee: President and Fellows of Harvard College
    Inventors: Amy J. Wagers, Jill Goldstein, Ryan G. Walker
  • Macrocyclic compound and uses thereof
    Patent number: 11725015
    Abstract: The present invention provides novel Compound (1) having tumor vascular remodeling effect and/or anti-CAF (Cancer Associated Fibroblasts) activity, or a pharmaceutically acceptable salt thereof, optionally in a pharmaceutically acceptable carrier, and medical uses thereof.
    Type: Grant
    Filed: February 4, 2021
    Date of Patent: August 15, 2023
    Assignees: President and Fellows of Harvard College, Eisai R&D Management Co., LTD.
    Inventors: Yoshito Kishi, Kazunobu Kira, Ken Ito
  • ANTERIOR NARES SWAB AND USES THEREOF
    Publication number: 20230248338
    Abstract: The technology described herein is directed to an anterior nares swab that is automation compatible. In one aspect, the swab comprises a cap, a threaded portion, a neck, and a sample collection head. The cap can be integrally and/or monolithically formed with any one or more of the threaded portion, the neck, and the sample collection head; or can be removably coupled to any one or more of the threaded portion, the neck, and the sample collection head. In additional aspects, described herein are kits comprising said swabs and methods of using said swabs.
    Type: Application
    Filed: July 13, 2021
    Publication date: August 10, 2023
    Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Michael SPRINGER, Richard NOVAK
  • Mass spectrometry technique for single cell proteomics
    Patent number: 11719703
    Abstract: The present invention generally provides, in various embodiments, methods of analyzing samples having a low abundance of proteins, e.g., single cells, utilizing liquid chromatography and tandem mass spectroscopy (LC-MS/MS).
    Type: Grant
    Filed: January 17, 2019
    Date of Patent: August 8, 2023
    Assignees: NORTHEASTERN UNIVERSITY, PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Bogdan Budnik, Nikolai Slavov, Harrison Specht, Ezra Levy
  • CRP capture/detection of gram positive bacteria
    Patent number: 11718651
    Abstract: Described herein are engineered microbe-targeting molecules, microbe-targeting articles, kits comprising the same, and uses thereof. Such microbe-targeting molecules, microbe-targeting articles, or the kits comprising the same can bind or capture of a microbe or microbial matter thereof, and can thus be used in various applications, such as diagnosis or treatment of an infection caused by microbes in a subject or any environmental surface.
    Type: Grant
    Filed: May 12, 2021
    Date of Patent: August 8, 2023
    Assignee: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Alexander L. Watters, Donald E. Ingber, Mark J. Cartwright, Michael Super, Martin Rottman, Evangelia Murray, Brendon Dusel
  • Engineered water nanostructures (EWNS) and uses thereof
    Patent number: 11718866
    Abstract: Various embodiments of the present invention relate to, among other things, systems for generating engineered water nanostructures (EWNS) comprising reactive oxygen species (ROS) and methods for inactivating at least one of viruses, bacteria, bacterial spores, and fungi in or on a wound of a subject in need thereof or on produce by applying EWNS to the wound or to the produce.
    Type: Grant
    Filed: August 2, 2022
    Date of Patent: August 8, 2023
    Assignee: President and Fellows of Harvard College
    Inventors: Philip Demokritou, Georgios Pyrgiotakis, Joseph D. Brain