Patents Assigned to President & Fellows of Harvard College
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Publication number: 20240108709Abstract: The present invention provides vaccine compositions and methods of producing such compositions. Other embodiments of the invention include methods of treating a pathogen infection, methods of vaccinating a subject against a pathogen infection, and methods for treating an antibiotic-resistance bacterial infection in a subject in need thereof. In further embodiments, the invention includes methods of decreasing the level of a pathogen in a subject having a pathogen infection, methods of increasing the surviving rate of a subject having a pathogen infection, methods of reducing the level of pain associated with a pathogen infection, and methods of reducing the level of distress associated with a pathogen infection in a subject in need thereof. Novel scaffold compositions and opsonin-bound or lectin-bound pathogen compositions, and uses thereof, are also provided herein.Type: ApplicationFiled: March 20, 2023Publication date: April 4, 2024Applicant: President and Fellows of Harvard CollegeInventors: Michael Super, Edward J. Doherty, Mark Joseph Cartwright, Des White, Alexander Stafford, Omar Abdel-Rahman Ali, Amanda Graveline, Donald E. Ingber, David J. Mooney, Benjamin Seiler
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Publication number: 20240110166Abstract: Provided herein are systems, compositions, and methods of introducing protective and/or loss-of-function variants of CCR5 and CCR2. Variants may be introduced using a CRISPR/Cas9-based nucleobase editor or other guide nucleotide sequence-programmable DNA binding protein domain-based fusion protein described herein. Further provided herein are compositions and methods of preventing and treating conditions related to HIV infection and progression as well as to AIDS.Type: ApplicationFiled: October 10, 2023Publication date: April 4, 2024Applicant: President and Fellows of Harvard CollegeInventors: Juan Pablo Maianti, David R. Liu
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Patent number: 11946055Abstract: Disclosed herein are methods, compositions, and kits for engineering proteins using error-prone orthogonal replication (epOrthoRep) and yeast surface display (YSD).Type: GrantFiled: December 9, 2021Date of Patent: April 2, 2024Assignees: The Regents of the University of California, President and Fellows of Harvard CollegeInventors: Chang C. Liu, Alon Wellner, Ziwei Zhong, Arjun Ravikumar, Andrew Kruse, Conor Thomas McMahon
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Patent number: 11944429Abstract: Systems and methods are disclosed to objectively identify sub-second behavioral modules in the three-dimensional (3D) video data that represents the motion of a subject. Defining behavioral modules based upon structure in the 3D video data itself— rather than using a priori definitions for what should constitute a measurable unit of action— identifies a previously-unexplored sub-second regularity that defines a timescale upon which behavior is organized, yields important information about the components and structure of behavior, offers insight into the nature of behavioral change in the subject, and enables objective discovery of subtle alterations in patterned action. The systems and methods of the invention can be applied to drug or gene therapy classification, drug or gene therapy screening, disease study including early detection of the onset of a disease, toxicology research, side-effect study, learning and memory process study, anxiety study, and analysis in consumer behavior.Type: GrantFiled: March 8, 2023Date of Patent: April 2, 2024Assignee: President and Fellows of Harvard CollegeInventors: Sandeep Robert Datta, Alexander B. Wiltschko, Matthew J. Johnson
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Patent number: 11946925Abstract: In a nanopore sensor, a nanopore disposed in a support structure has a nanopore diameter and nanopore resistance, RPore. A fluidic passage, disposed in fluidic connection between a first fluidic reservoir and the nanopore, has a cross-sectional extent, along at least a portion of the fluidic passage length, that is greater than the diameter of the nanopore and that is less than the fluidic passage length. The fluidic passage has a fluidic passage resistance, RFP, of at least about 10% of the nanopore resistance, RPore, and no more than about 10 times the nanopore resistance, RPore. The nanopore is disposed in fluidic connection between the fluidic passage and a second fluidic reservoir. At least one electrical transduction element is disposed at the fluidic passage and electrically connected to produce an indication of electrical potential local to the fluidic passage.Type: GrantFiled: April 12, 2022Date of Patent: April 2, 2024Assignee: President and Fellows of Harvard CollegeInventor: Ping Xie
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Publication number: 20240100767Abstract: In one aspect, the present disclosure provides a nozzle for a 3D printing system. The nozzle may include a flowpath with a material inlet and a material outlet. The nozzle may further include a valve in fluid communication with the flowpath between the material inlet and the material outlet, where the valve includes a closed state and an open state, where in the closed state the valve obstructs the flowpath between the material inlet and the material outlet, and where in the open state the material inlet is in fluid communication with the material outlet. The nozzle may further include a compensator in fluid communication with the flowpath, where the compensator includes a contracted state associated with the open state of the valve and an expanded state associated with the closed state of the valve.Type: ApplicationFiled: September 6, 2023Publication date: March 28, 2024Applicant: President and Fellows of Harvard CollegeInventors: Jennifer A. LEWIS, Mark A. Skylar-Scott, Jochen Mueller
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Publication number: 20240101967Abstract: Described herein are compositions and methods for generating a viable cell that expresses at least one or more woolly mammoth genes. Also described herein are compositions and methods for generating an embryo, blastula, oocyte, or non-human organism that expresses one or more woolly mammoth genes.Type: ApplicationFiled: December 10, 2021Publication date: March 28, 2024Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGEInventors: George M. Church, Eriona Hysolli, Jessica Weber, Pranam Chatterjee, Cory Smith
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Patent number: 11939571Abstract: Expressing guide nucleic acids (e.g., gRNA) from the same oligonucleotide that contains donor sequence permits the high efficiency, simultaneous transformation of a population of cells with both substrates. Using oligonucleotide chip array technology, one can construct thousands of oligonucleotides with customized gRNA and donor sequence in a cost effective manner. In combination, one can efficiently modify endogenous and exogenous genes.Type: GrantFiled: June 12, 2017Date of Patent: March 26, 2024Assignees: President and Fellows of Harvard College, The General Hospital CorporationInventors: Xiaoge Guo, Alejandro Chavez, Max Schubert, Eric Kelsic
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Patent number: 11940441Abstract: Provided herein relates to systems and methods for producing and using a body having a central channel separated by one or more membranes. The membrane(s) are configured to divide the central channel into at least one mesochannel and at least one microchannel. The height of the mesochannel is substantially greater than the height of the microchannel. A gaseous fluid can be applied through the mesochannel while a liquid fluid flowing through the microchannel. The systems and methods described herein can be used for various applications, including, e.g., growth and differentiation of primary cells such as human lung cells, as well as any other cells requiring low shear and/also stratified structures, or simulation of a microenvironment in living tissues and/or organs (to model physiology or disease states, and/or to identify therapeutic agents and/or vaccines). The systems and methods can also permit co-culture with one or more different cell types.Type: GrantFiled: September 11, 2020Date of Patent: March 26, 2024Assignee: President and Fellows of Harvard CollegeInventors: Donald E. Ingber, Kambez Hajipouran Benam, Remi Villenave, Geraldine A. Hamilton, Bryan Hassell, Christopher D. Hinojosa, Carolina Lucchesi
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Publication number: 20240094439Abstract: An optical component can include a substrate. The optical component can include a metasurface disposed on the substrate. The metasurface can include one or more linearly birefringent elements. A spatially-varying Jones matrix and a far-field of the metasurface can define a transfer function of the metasurface configured to generate a controlled response in the far-field according to polarization of light incident on the metasurface.Type: ApplicationFiled: December 27, 2021Publication date: March 21, 2024Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGEInventors: Noah A. RUBIN, Mohammad Aun Abbas ZAIDI, Federico CAPASSO
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Publication number: 20240093190Abstract: The present disclosure provides compositions and methods for inhibiting viral pathogenesis by targeting long noncoding ribonucleic acids.Type: ApplicationFiled: January 18, 2022Publication date: March 21, 2024Applicant: President and Fellows of Harvard CollegeInventors: Longlong Si, Haiqing Bai, Rachelle Pranti-Baun, Donald E. Ingber
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Publication number: 20240093156Abstract: The disclosure provides methods and compositions for reprogramming fibroblasts into limb progenitors.Type: ApplicationFiled: August 15, 2023Publication date: March 21, 2024Applicants: President and Fellows of Harvard College, The Brigham and Women's Hospital, Inc.Inventors: Clifford J. Tabin, Yuji Atsuta, Alan R. Rodrigues, ChangHee Lee, Olivier Pourquie
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Patent number: 11932884Abstract: Some aspects of this disclosure provide strategies, systems, reagents, methods, and kits that are useful for the targeted editing of nucleic acids, including editing a single site within the genome of a cell or subject, e.g., within the human genome. In some embodiments, fusion proteins comprise a Gam protein, a napDNAbp, and a cytidine deaminase. In some embodiments, the fusion proteins further comprise a UGI domain. In some embodiments, methods for targeted nucleic acid editing are provided. In some embodiments, reagents and kits for the generation of targeted nucleic acid editing proteins, e.g., fusion proteins of a Gam protein, a cytidine deaminase and nucleic acid editing proteins or domains, are provided.Type: GrantFiled: March 21, 2022Date of Patent: March 19, 2024Assignee: President and Fellows of Harvard CollegeInventors: David R. Liu, Kevin Tianmeng Zhao, Yongjoo Kim
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Publication number: 20240082420Abstract: Methods, systems, compositions and strategies for the delivery of RNA into cells in vivo, ex vivo, or in vitro via ARMMs are provided. In some aspects, ARMMs containing fusion proteins of ARRDC1 fused to an RNA binding protein or an RNA binding protein fused to a WW domain are provided. In some aspects, ARMMs containing binding RNAs associated with cargo RNAs are provided. In other aspects, cargo RNAs associated with a binding RNA, such as a TAR element, are loaded into ARMMs via ARRDC1 fusion proteins containing an RNA binding protein, such as trans-activator of transcription (Tat) protein.Type: ApplicationFiled: June 28, 2023Publication date: March 14, 2024Applicant: President and Fellows of Harvard CollegeInventor: Quan Lu
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Publication number: 20240084364Abstract: The invention provides for systems, methods, and compositions for targeting nucleic acids. In particular, the invention provides non-naturally occurring or engineered DNA-targeting systems comprising a novel DNA-targeting CRISPR effector protein and at least one targeting nucleic acid component like a guide RNA Methods for making and using and uses of such systems, methods, and compositions and products from such methods and uses are also disclosed and claimed.Type: ApplicationFiled: August 17, 2023Publication date: March 14, 2024Applicants: The Broad Institute, Inc., Massachusetts Institute of Technology, President and Fellows of Harvard CollegeInventors: Feng ZHANG, Bernd ZETSCHE, Jonathan S. GOOTENBERG, Omar O. ABUDAYYEH, Ian SLAYMAKER
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Patent number: 11925933Abstract: The present invention generally relates to microfluidic devices. In some aspects, various entities, such as droplets or particles, may be contained within a microfluidic device, e.g., within collection chambers or other locations within the device. In some cases, the entities may be released from such locations, e.g., in a sequential pattern, or an arbitrary pattern. In some cases, the entities may be imaged, reacted, analyzed, etc. while contained within the collection chambers. Other aspects are generally directed to methods of making or using such devices, kits involving such devices, or the like.Type: GrantFiled: April 14, 2017Date of Patent: March 12, 2024Assignee: President and Fellows of Harvard CollegeInventors: David A. Weitz, Kiryakos S. Mutafopulos, Thomas Hufnagel
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Publication number: 20240076652Abstract: The disclosure provides adenosine deaminases that are capable of deaminating adenosine in DNA. The disclosure also provides fusion proteins comprising a Cas9 (e.g., a Cas9 nickase) domain and adenosine deaminases that deaminate adenosine in DNA. In some embodiments, the fusion proteins further comprise a nuclear localization sequence (NLS), and/or an inhibitor of base repair, such as, a nuclease dead inosine specific nuclease (dISN).Type: ApplicationFiled: February 24, 2023Publication date: March 7, 2024Applicant: President and Fellows of Harvard CollegeInventors: David R. Liu, Nicole Gaudelli
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Publication number: 20240077502Abstract: Evolved sortases exhibiting enhanced reaction kinetics and/or altered substrate preferences are provided herein, for example evolved sortases that bind recognitions motifs comprising a LMVGG [SEQ ID NO: 3] sequence. Also provided are methods (e.g., orthogonal transpeptidation and diagnostics methods) for using such sortases.Type: ApplicationFiled: January 11, 2022Publication date: March 7, 2024Applicants: The Broad Institute, Inc., President and Fellows of Harvard College, The Brigham and Women's Hospital Inc.Inventors: David R. Liu, Christopher J Podracky, Chihui An, Dominic Walsh
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Publication number: 20240075012Abstract: The present disclosure provides compositions and methods for inhibiting respiratory viral infections, inflammatory diseases, and/or respiratory inflammation.Type: ApplicationFiled: August 11, 2023Publication date: March 7, 2024Applicant: President and Fellows of Harvard CollegeInventors: Haiqing Bai, Longlong Si, Rachelle Prantil-Baun, Donald E. Ingber
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Patent number: 11920150Abstract: Described herein are targeting moieties that can be capable of specifically targeting muscle cells and can include an n-mer motif. In some embodiments, the n-mer motif contains an RGD motif. Also described herein are vector systems, particles, polypeptides that can encode and/or contain one or more targeting moieties. Also described herein are methods of delivering a cargo to a cell, such as a muscle cell, using one or more of the targeting moieties described herein.Type: GrantFiled: March 29, 2022Date of Patent: March 5, 2024Assignees: The Broad Institute, Inc., President and Fellows of Harvard College, Massachusetts Institute of TechnologyInventors: Pardis Sabeti, Mohammadsharif Tabebordbar, Simon Ye