Abstract: A pH-modified luminescent composition and methods. In composition, a marker exhibiting luminescence change over a indicatory period. pH-based luminesce change may be in intensity or in wavelength. One embodiment provides a luminescent composition, including a selected luminescent marker having a first phase; and a selected ionizing agent comprising a second phase. The selected luminescent marker exhibits luminescence for an indicatory period responsive to intermixing of the first phase with the second phase. A selected antimicrobial agent combined with the first phase of the luminescent composition, wherein intermixing of the first phase with the second phase is at least a portion of a hand hygiene protocol.

Abstract: A luminescent composition, with a luminescent marker and a ionizing agent exhibiting luminescence for an indicatory period once intermixed, and with luminance intensity remaining at a threshold intensity during indicatory period. Luminescence also can be exhibited responsive to responsive to excitatory light applied to the composition. The luminescent marker includes a coumarinic compound and the ionizing agent includes an ammonium base. Antimicrobial agents are included. A luminescence measuring apparatus includes a photoemitter and a photodetector responsive to an emissive light induced by photoemitter. An optical filter can be used with the photodetector. A two-phase composition dispensing apparatus includes a first phase reservoir, a second phase reservoir, a mixing nozzle, and a dispensing mechanism.

Abstract: A novel process for removing endotoxin from biological fluids such as parenteral fluids and for removing or reducing the level of endotoxin from the blood of animals is disclosed. The novel process includes the utilization of certain non-ionogenic hydrophobic synthetic plastic polymers that have been found to be capable of adsorbing endotoxin from the biological fluids when placed in intimate contact therewith.

Abstract: A novel process for removing endotoxin from biological fluids such as parenteral fluids and for removing or reducing the level of endotoxin from the blood of animals is disclosed. The novel process includes the utilization of certain non-ionogenic hydrophobic synthetic plastic polymers that have been found to be capable of adsorbing endotoxin from the biological fluids when placed in intimate contact therewith.

Abstract: An in vitro process for detecting the presence of endotoxin in a biological fluid such as a parenteral fluid, whole blood, and the like, wherein amebocyte lysate from the hemolymph of the horseshoe crab, Limulus polyphemus, is intimately contacted with and incubated in the presence of a synthetic plastic polymer capable of adsorbing endotoxin which has previously been contacted with the biological fluid. The presence or absence of a gelation reaction in the amebocyte lysate is then observed. The presence of a gelation reaction determines the presence of endotoxin in the biological fluid.