Abstract: A method for producing high-concentration stem cell exosomes with enhanced anti-inflammatory function using Lipopolysaccharide (LPS) and/or Lipoteichoic Acid (LTA), as well as anti-inflammatory and/or regenerative compositions that can be produced by the aforementioned method are disclosed. The production method for the anti-inflammatory composition includes a first step of treating stem cells during cultivation with one or more selected from a group consisting of Lipopolysaccharide (LPS) and Lipoteichoic Acid (LTA), and optionally, a second step of separating stem cells, their culture medium, and/or their exosomes obtained as a result of the first step.

Abstract: A method for isolating and culturing umbilical cord blood stem cells that express high levels of GDF-3 is disclosed. Umbilical cord blood stem cells isolated and cultured as described herein exhibit high expression of GDF-3. Stem cells with high expression of GDF-3 maintain high proliferation capacity contrary to conventional stem cells even as passages progress, allowing for the advantage of obtaining a large quantity of healthy stem cells. Furthermore, due to the higher proliferation rate of stem cells, it has been observed that the concentration of extracellular matrix components secreted by the cells is also higher. Therefore, when utilizing GDF-3 high-expressing umbilical cord blood stem cells for the production of cell and culture medium compositions, it is possible to produce raw materials with excellent efficacy and effects.