Abstract: A method of collecting one or more target macromolecules in a capture membrane by gel electrophoresis is disclosed, as well as a kit for macromolecule isolation and recovery including: a preformed gel; a capture device; an insertion guide; and optionally, a migration gauge.
Abstract: A device which can be used for filtering samples is described. The device is comprised of a plurality of filter wells, each having a wide region and a narrow region. The device is fitted with a filter element and then filled with a gel filtration matrix which is selected so that molecules of a particular molecular size will diffuse into the matrix. The device is centrifuged to remove water from the interstitial regions of the gel filtration matrix, and a sample is then added to the device containing the dried gel filtration matrix. The device is again centrifuged, causing smaller molecules to diffuse into the matrix and allowing larger molecules to exit the device, wherein they are collected and analyzed. The device is capable of filtering sample sizes as low as 1 &mgr;L and can be used in a variety of existing laboratory equipment, including bench-top centrifuges and microcentrifuges. A process for using the device is also disclosed.
Type:
Grant
Filed:
September 20, 2000
Date of Patent:
June 11, 2002
Assignee:
Princeton Separations
Inventors:
Paul T. Nix, Dennis R. Stocker, Michael J. Byers