Abstract: Disclosed is a method of producing a compound with &bgr;1-4 linkage which contains the lactosamine structure involving reacting at least one donor substance Gal&bgr;OR where R is an organic group, and at least one acceptor substance which is a glucopyranosamino derivative having the formula GlcNR″—R′″, wherein NR″ is an azido, 2-N-acetyl-, 2-N-phtalimido, or an organic group bound to the 2-N-group of glucosamine, wherein R′″ is a glycosidically bound fluoro or is an O-, C-, N- or S-glycosidically bound aliphatic or aromatic compound, with the proviso that if NR″ is NHAc then R′″ is not OH and if NR″ is not NHAc then R′″ may be OH, in the presence of Bullera singularis or an E.C. group 3.2 glycosidase of essentially the same structure as an E.C. Group 3.
Abstract: The present invention relates to simplified synthesis, new carbohydrate-based products and practical use of different carbohydrate-based products. Examples of these are (Gal&agr;1-3Gal), GlcNAc&bgr;1-3Gal, &agr;- or &bgr;-glycosides thereof, Gal&agr;1-3Gal- containing tri-, or higher oligosaccharides, &agr;- or &bgr;-glycosides thereof, GlcNAc&bgr;1-3Gal containing tri-, tetra-, or higher oligosaccharides, and derivatives and/or &agr;- or &bgr;-glycosides thereof, Gal&agr;1-3GalGlcNAc&bgr;1-3Gal, &agr;- or &bgr;-glycosides thereof, Gal&agr;1-3Gal&bgr;1-4GlcNAc&bgr;1-3Gal&bgr;1-4Glc, or other higher oligosaccharides containing the Gal&agr;1-3Gal-structure, &agr;- or &bgr;-glycosides thereof, modified carbohydrates, di-, tri-, oligo-, or polyfunctional products containing carbohydrate structures, and the use of the products for synthesis, affinity purification, diagnostic applications and therapy.
Abstract: A method for synthesis of oligosaccharide compounds which either consist of or are fragments or analogs of the carbohydrate part in glycoconjugates is described. The synthesis from donor and acceptor substrates is carried out in that at least one glycosidase and at least one glycosyltransferase are used as catalysts and the oligosaccharide compound is isolated from the reaction mixture.
Abstract: The present invention relates to simplified synthesis, new carbohydrate-based products and practical use of different carbohydrate-based products. Examples of these are (Gal?1-3Gal), GlcNAc?1-3Gal, ?- or ?-glycosides thereof, Gal?1-3Gal- containing tri-, or higher oligosaccharides, ?- or ?-glycosides thereof, GlcNAc?1-3Gal containing tri-, tetra-, or higher oligosaccharides, and derivatives and/or ?- or ?-glycosides thereof, Gal?1-3GalGlcNAc?1-3Gal, ?- or ?-glycosides thereof, Gal?1-3Gal?1-4GlcNAc?1-3Gal?1-4Glc, or other higher oligosaccharides containing the Gal?1-3Gal-structure, ?- or ?-glycosides thereof, modified carbohydrates, di-, tri-, oligo-, or polyfunctional products containing carbohydrate structures, and the use of the products for synthesis, affinity purification, diagnostic applications and therapy.