Abstract: Plants are produced that express an amino acid sequence that, when administered to a fish, produce an antigenic or immune response in the fish. The amino acid sequence in one embodiment is an antigen from an organism that causes pathology in fish. The plant tissue may be fed to the fish, or mixed with other materials and fed to fish, or extracted and administered to the fish.
Abstract: A method for extraction of heterologous protein from monocotyledonous plant seed comprises extracting the germ portion of the seed and extracting and purifying the protein from the germ. Enhanced expression in the germ is provided, and allows for improved efficiency in production, and cost savings. Directing expression to the germ portion further increases expression levels of the protein. The ubiquitin promoter preferentially directs expression to the germ portion of plant seed.
Abstract: Expression of a recombinant manganese-dependent peroxidase in plants is described. Methods for the recovery of transgenic corn seeds that express high levels of functional manganese-dependent peroxidase are also provided. Manganese-dependent peroxidase vectors are engineered to contain sequences directing seed-preferred expression as well as sequences for cell wall-targeted localization.
Type:
Grant
Filed:
June 6, 2003
Date of Patent:
June 27, 2006
Assignees:
Prodi Gene, Inc., Geneucor International, Inc.
Inventors:
Elizabeth Hood, John Howard, Richard Clough, Kameshwari Pappu
Abstract: Production of proteases in plants is set forth, whereby heterologous DNA encoding the protease is introduced into the plant and expression of the protein achieved. By such methods, expression is achieved in plants wherein the plant cell is not damaged, the protein can be recovered without contamination by other proteases, and can be expressed at levels such that commercial production of the enzyme is obtained. Expression levels can be at 0.1% of total soluble protein of the plant, or higher.
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