Patents Assigned to Prolinx Incorporated
  • Removal of dye-labeled dideoxy terminators from DNA sequencing reactions
    Patent number: 6818760
    Abstract: This invention provides methods for removing unincorporated dye-labeled molecules from a mixture that includes dye-labeled polynucleotides or other polymers and the unincorporated dye-labeled molecules. The methods involve adsorbing the unincorporated dye-labeled molecules into a plurality of particles that are made up of one or more porous hydrophobic materials that are encapsulated in a hydrophilic matrix.
    Type: Grant
    Filed: October 6, 2000
    Date of Patent: November 16, 2004
    Assignee: Prolinx Incorporated
    Inventors: Douglas A. Spicer, Karin A. Hughes, Robert J. Kaiser, James E. Mahoney, Amy L. Springer, Mark L. Stolowitz, Carl H. D. Weissman
  • Sensor surfaces for detecting analytes and methods of use
    Publication number: 20030032202
    Abstract: The present invention provides a method for detecting an analyte, comprising providing a sensor comprising a substrate coated with a free electron metal, a matrix layer disposed on the free electron metal, the matrix layer comprising an organic compound, wherein the organic compound has a boronic acid complexing moiety and, a boronic acid moiety complexed to the boronic acid complexing moiety; and contacting the sensor with the analyte to elicit a response; and measuring the response thereby detecting the analyte.
    Type: Application
    Filed: April 2, 2002
    Publication date: February 13, 2003
    Applicant: Prolinx Incorporated
    Inventors: Mark L. Stolowitz, Jean P. Wiley, Guisheng Li, Kevin Lund
  • Systems and apparatus for the analysis of molecular interactions
    Publication number: 20030003018
    Abstract: Instruments and systems for the analysis of molecular interactions with enhanced throughput and ease-of-use. In certain aspects, the systems and instruments include miniaturized SPR-based sensors and novel sensor surface chemistry to provide high-throughput automated instruments and systems for molecular interaction analysis.
    Type: Application
    Filed: April 2, 2002
    Publication date: January 2, 2003
    Applicant: Prolinx Incorporated
    Inventors: Mark L. Stolowitz, Robert J. Kaiser, Leslie E. Linkkila, Charles E. Dubuque, Michael E. Baum, Erik M. Engstrom, Stephen M. Bailey
  • Sensor surfaces for detecting analytes
    Publication number: 20020192722
    Abstract: The present invention provides a sensor surface comprising: a substrate coated with a free electron metal; and a matrix layer disposed on the free electron metal, wherein the matrix layer comprises an organic compound having a boronic acid complexing moiety. The matrix is preferably a self-assembled monolayer (SAM), a mixed self-assembled monolayer (mSAM), or combinations thereof.
    Type: Application
    Filed: April 2, 2002
    Publication date: December 19, 2002
    Applicant: Prolinx Incorporated
    Inventors: Mark L. Stolowitz, Jean P. Wiley, Guisheng Li, Kevin Lund
  • Boronic acid containing phosphoramidite reagents
    Patent number: 6031117
    Abstract: The present invention provides arylboronic acid reagents useful for incorporation into modified oligonucleotides and polynucleotides. The modified oligonucleotides and polynucleotides thus produced are useful in bioconjugation reactions for the immobilization and purification of macromolecules.
    Type: Grant
    Filed: March 19, 1999
    Date of Patent: February 29, 2000
    Assignee: Prolinx Incorporated
    Inventors: Robert J. Kaiser, Mark L. Stolowitz
  • Boronic acid containing oligonucleotides and polynucleotides
    Patent number: 6013783
    Abstract: The present invention relates to the field of nucleic acid immobilization, purification and detection and, more particularly, to boronic acid modified oligonucleotides and polynucleotides useful in bioconjugation reactions. The modified oligonucleotides and polynucleotides are useful in reactions for the immobilization and purification of macromolecules.
    Type: Grant
    Filed: March 19, 1999
    Date of Patent: January 11, 2000
    Assignee: Prolinx Incorporated
    Inventors: Robert J. Kaiser, Mark L. Stolowitz
  • Use of boron-containing polynucleotides as diagnostic agents
    Patent number: 5876938
    Abstract: Modified nucleotides and polynucleotides which are useful in hybridization assays for the detection of target genes are provided. The modified polynucleotides contain at least one boronic acid moiety which is attached to a nucleotide base in a position which does not interfere with the hydrogen bonding capabilities of that base during duplex formation. The modified polynucleotides are typically formed from naturally occurring nucleotides and one or more modified nucleotides.
    Type: Grant
    Filed: April 11, 1997
    Date of Patent: March 2, 1999
    Assignee: Prolinx, Incorporated
    Inventors: Mark L. Stolowitz, Robert J. Kaiser
  • Boronic acid-contaning nucleic acid monomers
    Patent number: 5831046
    Abstract: Modified nucleotides and polynucleotides which are useful in hybridization assays for the detection of target genes are provided. The modified polynucleotides contain at least one boronic acid moiety which is attached to a nucleotide base in a position which does not interfere with the hydrogen bonding capabilities of that base during duplex formation. The modified polynucleotides are typically formed from naturally occurring nucleotides and one or more modified nucleotides.
    Type: Grant
    Filed: August 5, 1996
    Date of Patent: November 3, 1998
    Assignee: Prolinx, Incorporated
    Inventors: Mark L. Stolowitz, Robert J. Kaiser