Patents Assigned to Prolume, LTD
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Patent number: 11965122Abstract: Calcium reacting photoproteins are a class of self-illuminating proteins that emit light upon contact with calcium. Fluorescent Proteins are small proteins that change the color of light when excited. Fluorescent Proteins and Calcium Activated Photoproteins can be used to enhance, dazzle, amaze, startle, and otherwise entertain an audience by their direct application on to the audience, surroundings, the actors, or sprayed on settings as in the newer 4D movies. The disclosure comprises novel Coelenterazine compounds and methods of use, including a simple delivery device for the photoprotein to create unique and novel cinematic, theatrical, stage, movie and musical concert optical effects by their luminous reaction upon contact with surfaces that contain calcium. Calcium is ubiquitous in and on most surfaces and in the environment; it is this unique property of calcium that makes this a novel use of the photoproteins for entertainment. A generic formula of Coelenterazine as shown.Type: GrantFiled: June 10, 2021Date of Patent: April 23, 2024Assignee: PROLUME, LTD.Inventors: Bruce Bryan, Hendrik Schmidt, Millard Cull
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Patent number: 11034886Abstract: Novel compounds in the classes of Imidazo[1,2-?]pyrazines and Imidazo[1,2-?]quinoxalines with a methoxy group modification show unique spectral properties when used as a substrate for luciferases and photo-proteins. Methoxy e-Coelenterazine, an Imidazo[1,2-?] quinoxaline shifts the emission wavelength by 80 nm to blue light compared to e-Coelenterazine, using Renilla reniformis luciferase. The novel analogues described here are useful in combination with fluorescent proteins to create BRET (bioluminescent resonance energy transfer) and for other luminescent assays. In addition these novel compounds can be used to enhance, dazzle, amaze, startle, and otherwise entertain an audience by their direct application on to the audience, surroundings, the actors, or sprayed on settings as in the newer 4D movies.Type: GrantFiled: April 8, 2019Date of Patent: June 15, 2021Assignee: Prolume, LTD.Inventors: Bruce Bryan, Hendrik Schmidt, Millard Cull
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Patent number: 10562773Abstract: The present invention relates to the extraordinary properties of recently discovered nanotubes. This disclosure teaches a method for using barrel proteins acting as scaffolds to guide assembly of nanotubes, and using nano-molecular molding jigs to format the nanotubes into stable arrays with the precise geometric architecture desired. This disclosure teaches nanotube technology with principles of protein folding and aggregated self-assembly. In certain embodiments, the disclosure teaches using highly modified barrel proteins to form hydrophobic and hydrophilic channels that guide the nanotubes into their centers, or other geometric patterns utilizing silicone aerogel to form nano-molecular molds, jigs, and surfaces to position nanotubes in precise geometric arrangements and arrays. This disclosure teaches new uses of barrel proteins as self-assembling molding tools to develop new nanometer scaled devices and their uses herein.Type: GrantFiled: July 17, 2018Date of Patent: February 18, 2020Assignee: PROLUME, LTD.Inventor: Bruce Bryan
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Patent number: 10253254Abstract: The disclosure comprises novel Coelenterazine compounds and methods of use, including a simple delivery device for the photoprotein to create effects by their luminous reaction upon contact with surfaces that contain calcium. Calcium is ubiquitous in and on most surfaces and in the environment; it is this unique property of calcium that makes this a novel use of the photoproteins for entertainment. A base coelenterazine structure is depicted below.Type: GrantFiled: April 18, 2017Date of Patent: April 9, 2019Assignee: PROLUME, LTD.Inventors: Bruce Bryan, Hendrik Schmidt, Millard Cull
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Patent number: 10071911Abstract: The present invention relates to the extraordinary properties of recently discovered nanotubes. This disclosure teaches a method for using barrel proteins acting as scaffolds to guide assembly of nanotubes, and using nano-molecular molding jigs to format the nanotubes into stable arrays with the precise geometric architecture desired. This disclosure teaches nanotube technology with principles of protein folding and aggregated self-assembly. In certain embodiments, the disclosure teaches using highly modified barrel proteins to form hydrophobic and hydrophilic channels that guide the nanotubes into their centers, or other geometric patterns utilizing silicone aerogel to form nano-molecular molds, jigs, and surfaces to position nanotubes in precise geometric arrangements and arrays. This disclosure teaches new uses of barrel proteins as self-assembling molding tools to develop new nanometer scaled devices and their uses herein.Type: GrantFiled: March 5, 2014Date of Patent: September 11, 2018Assignee: PROLUME, LTD.Inventor: Bruce Bryan
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Patent number: 9624425Abstract: Novel compounds in the classes of Imidazo[1,2-?]pyrazines and Imidazo[1,2-?]quinoxalines with a methoxy group modification show unique spectral properties when used as a substrate for luciferases and photo-proteins. Methoxy e-Coelenterazine an Imidazo[1,2-?] quinoxaline shifts the emission wavelength by 80 nm to blue light compared to e-Coelenterazine, using Renilla reniformis luciferase. The novel analogs described here are useful in combination with fluorescent proteins to create BRET (bioluminescent resonance energy transfer) and for other luminescent assays. In addition these novel compounds can be used in novelty items and for entertaining purposes to create different light emitting colors using the same luciferase or photoprotein.Type: GrantFiled: August 30, 2013Date of Patent: April 18, 2017Assignee: Prolume, LTD.Inventors: Bruce Bryan, Hendrik Schmidt, Millard Cull
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Patent number: 9115364Abstract: The present invention relates to a method of producing a target protein, which method comprises expressing said protein in a host cell which contains a nucleic acid molecule which encodes a chimeric protein, said chimeric protein comprising a signal peptide from a non-mammalian bulk-secreted protein and said target protein; nucleic acids, vectors, host cells and kits for carrying out the method are also described.Type: GrantFiled: November 28, 2011Date of Patent: August 25, 2015Assignee: PROLUME LTD.Inventors: John Hesketh, Hanne Ravneberg, Christine Gjerdrum, Albert Tauler, Ian Pryme, Beate Stern
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Patent number: 8067198Abstract: The present invention relates to a method of producing a target protein, which method comprises expressing said protein in a host cell which contains a nucleic acid molecule which encodes a chimeric protein, said chimeric protein comprising a signal peptide from a non-mammalian bulk-secreted protein and said target protein; nucleic acids, vectors, host cells and kits for carrying out the method are also described.Type: GrantFiled: June 25, 2004Date of Patent: November 29, 2011Assignee: Prolume Ltd.Inventors: John Hesketh, Hanne Ravneberg, Christine Gjerdrum, Albert Tauler, Ian Pryme, Beate Stern
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Patent number: 7238497Abstract: Synthetic versions of a full length and termini truncated humanized green fluorescent protein based on Ptilosarcus gurneyi are disclosed which have been modified to the favored or most favored codons for mammalian expression systems. The disclosed encoded protein has 239 amino acid residues compared with the wild type Ptilosarcus gurneyi which has 238 amino acids. In the present invention, a valine residue has been added at the second position from the amino terminus and codon preference bias has been changed in a majority of the wild type codons of Ptilosarcus gurneyi fluorescent protein. The humanized Ptilosarcus gurneyi green fluorescent protein is useful as a fluorescent tag for monitoring the activities of its fusion partners using imaging based approaches.Type: GrantFiled: July 14, 2004Date of Patent: July 3, 2007Assignee: Prolume, Ltd.Inventors: Yih-Tai Chen, Longguang Cao
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Publication number: 20060183894Abstract: Synthetic versions of a full length and termini truncated humanized green fluorescent protein based on Ptilosarcus gurneyi are disclosed which have been modified to the favored or most favored codons for mammalian expression systems. The disclosed encoded protein has 239 amino acid residues compared with the wild type Ptilosarcus gurneyi which has 238 amino acids. In the present invention, a valine residue has been added at the second position from the amino terminus and codon preference bias has been changed in a majority of the wild type codons of Ptilosarcus gurneyi fluorescent protein. The humanized Ptilosarcus gurneyi green fluorescent protein is useful as a fluorescent tag for monitoring the activities of its fusion partners using imaging based approaches.Type: ApplicationFiled: April 25, 2006Publication date: August 17, 2006Applicant: Prolume Ltd.Inventors: Yih-Tai Chen, Longguang Cao
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Patent number: 7045599Abstract: Synthetic versions of a full length and termini truncated humanized green fluorescent protein based on Ptilosarcus gurneyi are disclosed which have been modified to the favored or most favored codons for mammalian expression systems. The disclosed encoded protein has 239 amino acid residues compared with the wild type Ptilosarcus gurneyi which has 238 amino acids. In the present invention, a valine residue has been added at the second position from the amino terminus and codon preference bias has been changed in a majority of the wild type codons of Ptilosarcus gurneyi fluorescent protein. The humanized Ptilosarcus gurneyi green fluorescent protein is useful as a fluorescent tag for monitoring the activities of its fusion partners using imaging based approaches.Type: GrantFiled: July 14, 2004Date of Patent: May 16, 2006Assignee: Prolume Ltd.Inventors: Yih-Tai Chen, Longguang Cao
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Patent number: 6682899Abstract: Solid phase methods for the identification of an analyte in a biological medium, such as a body fluid, using bioluminescence are provided. A chip designed for performing the method and detecting the bioluminescence is also provided. Methods employing biomineralization for depositing silicon on a matrix support are also provided. A synthetic synapse is also provided.Type: GrantFiled: April 19, 2002Date of Patent: January 27, 2004Assignee: Prolume, Ltd.Inventors: Bruce J. Bryan, Stephen Gaalema, Randall B. Murphy
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Patent number: 6649357Abstract: Solid phase methods for the identification of an analyte in a biological medium, such as a body fluid, using bioluminescence are provided. A chip designed for performing the method and detecting the bioluminescence is also provided. Methods employing biomineralization for depositing silicon on a matrix support are also provided. A synthetic synapse is also provided.Type: GrantFiled: April 19, 2002Date of Patent: November 18, 2003Assignee: Prolume, Ltd.Inventors: Bruce J. Bryan, Stephen Gaalema, Randall B. Murphy
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Patent number: 6649356Abstract: Solid phase methods for the identification of an analyte in a biological medium, such as a body fluid, using bioluminescence are provided. A chip designed for performing the method and detecting the bioluminescence is also provided. Methods employing biomineralization for depositing silicon on a matrix support are also provided. A synthetic synapse is also provided.Type: GrantFiled: April 19, 2002Date of Patent: November 18, 2003Assignee: Prolume, Ltd.Inventors: Bruce J. Bryan, Stephen Gaalema, Randall B. Murphy
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Patent number: 6596257Abstract: Kits containing the diagnostic systems and diagnostic systems that rely on bioluminescence for visualizing tissues in situ are provided. The systems include compositions containing conjugates that include a tissue specific, particularly a tumor-specific, targeting agent linked to a targeted agent, a luciferase or luciferin. The systems also include a second composition that contains the remaining components of a bioluminescence generating reaction. Administration of the compositions results production of light by targeted tissues that permits the detection and localization of neoplastic tissue for surgical removal.Type: GrantFiled: December 22, 2000Date of Patent: July 22, 2003Assignee: Prolume, Ltd.Inventor: Bruce Bryan
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Patent number: 6458547Abstract: Solid phase methods for the identification of an analyte in a biological medium, such as a body fluid, using bioluminescence are provided. A chip designed for performing the method and detecting the bioluminescence is also provided. Methods employing biomineralization for depositing silicon on a matrix support are also provided. A synthetic synapse is also provided.Type: GrantFiled: December 12, 1997Date of Patent: October 1, 2002Assignee: Prolume, Ltd.Inventors: Bruce J. Bryan, Stephen Gaalema, Randall B. Murphy
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Patent number: 6436682Abstract: Isolated and purified nucleic acid molecules that encode a luciferase from Renilla mulleri, Gaussia and Pleuromamma, and the proteins encoded thereby are provided. Isolated and purified nucleic acids encoding green fluorescent proteins from the genus Renilla and Ptilosarcus, and the green fluorescent proteins encoded thereby are also provided. Compositions and combinations comprising the green fluorescent proteins and/or the luciferase are further provided.Type: GrantFiled: June 30, 2000Date of Patent: August 20, 2002Assignee: Prolume, Ltd.Inventors: Bruce J. Bryan, Christopher Szent-Gyorgyi
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Patent number: 6416960Abstract: Diagnostic systems that rely on bioluminescence for visualizing tissues in situ are provided. The systems are particularly useful for visualizing and detecting neoplastic tissue and specialty tissue during surgical procedures. Kits that provide the components of the systems and methods using the systems for visualizing the tissue are also provided. The systems include compositions containing conjugates that include a tissue specific, particularly a tumor-specific, targeting agent linked to a targeted agent, a luciferase or luciferin. The systems also include a second composition that contains the remaining components of a bioluminescence generating reaction. Administration of the compositions results production of light by targeted tissues that permits the detection and localization of neoplastic tissue for surgical removal. Therapeutic methods in which photosensitizing compounds are administered are also provided.Type: GrantFiled: August 8, 1997Date of Patent: July 9, 2002Assignee: Prolume, Ltd.Inventor: Bruce Bryan
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Patent number: 6232107Abstract: Isolated and purified nucleic acid molecules that encode a luciferase from Renilla mulleri, Gaussia and Pleuromamma, and the proteins encoded thereby are provided. Isolated and purified nucleic acids encoding green fluorescent proteins from the genus Renilla and Ptilosarcus, and the green fluorescent proteins encoded thereby are also provided. Compositions and combinations comprising the green fluorescent proteins and/or the luciferase are further provided.Type: GrantFiled: March 26, 1999Date of Patent: May 15, 2001Assignees: Prolume, LTDInventors: Bruce J. Bryan, Christopher Szent-Gyorgyi