Patents Assigned to Promega Corp.
  • Methods and kits for transferases
    Patent number: 7314729
    Abstract: A method for detecting transferase activity of a sample includes contacting the sample with a substrate and at least one of a phosphate group donor and a phosphate group acceptor. The substrate includes a reporter compound and amino acids. A peptidase is added that cleaves a non-phosphorylated substrate at a first rate and a phosphorylated substrate and a second rate. The output of the reporter compound is detected. In a preferred embodiment, the transferase activity detected is a kinase activity. In another preferred embodiment, the transferase activity detected is a phosphatase activity. Also provided is a method of screening for alterations in a transferase reaction. Kits and peptide substrate are also provided for carrying out at least one of the methods of the invention.
    Type: Grant
    Filed: June 3, 2004
    Date of Patent: January 1, 2008
    Assignee: Promega Corp.
    Inventors: Said A. Goueli, Robert F. Bulleit
  • Methods and kits for transferases
    Patent number: 7195884
    Abstract: A method for detecting transferase activity of a sample includes contacting the sample with a substrate and at least one of a phosphate group donor and a phosphate group acceptor. The substrate includes a reporter compound and amino acids. A peptidase is added that cleaves a non-phosphorylated substrate at a first rate and a phosphorylated substrate and a second rate. The output of the reporter compound is detected. In a preferred embodiment, the transferase activity detected is a kinase activity. In another preferred embodiment, the transferase activity detected is a phosphatase activity. Also provided is a method of screening for alterations in a transferase reaction. Kits and peptide substrate are also provided for carrying out at least one of the methods of the invention.
    Type: Grant
    Filed: July 19, 2002
    Date of Patent: March 27, 2007
    Assignee: Promega Corp.
    Inventors: Said A. Goueli, Robert F. Bulleit
  • Antibodies to cytokines in the prevention and treatment of inflammatory bowel disease
    Patent number: 6663864
    Abstract: Methods are described for treating inflammatory bowel disease in animals, including humans. Specific avian polyclonal antibodies directed to proinflammatory cytokines (such as IL-6 and TNF) are shown to have a beneficial effect in animal models predictive of human therapy for the treatment of colitis, including Crohn's disease.
    Type: Grant
    Filed: June 4, 1999
    Date of Patent: December 16, 2003
    Assignee: Promega Corp.
    Inventors: John A. Kink, Katherine L. Worledge, Douglas C. Stafford
  • Polymerase stabilization by polyethoxylated amine surfactants
    Patent number: 6242235
    Abstract: The present invention provides methods and compositions for protein stabilization, particularly the stabilization of polymerases in aqueous solutions with cationic surfactants. The present invention further provides cationic surfactants, including polyethoxylated amines, that stabilize thermostable and thermolabile enzymes in solution. These surfactants stabilize the activity of various enzymes, including thermostable DNA polymerases, thermolabile DNA polymerases and reverse transcriptases.
    Type: Grant
    Filed: June 22, 1999
    Date of Patent: June 5, 2001
    Assignee: Promega Corp.
    Inventors: John W. Shultz, Fen Huang