Patents Assigned to Promega Corporation
  • Patent number: 11293047
    Abstract: Provided herein are isolated polynucleotide encoding modified click beetle luciferase polypeptides that have enhanced luminescence and longer wavelength near-infrared signals. The disclosure also relates to near-infrared bioluminescence systems that include said modified click beetle luciferase polypeptides and novel luciferin derivatives, as well as methods of using said modified click beetle luciferase polypeptides and bioluminescence systems.
    Type: Grant
    Filed: December 23, 2019
    Date of Patent: April 5, 2022
    Assignee: Promega Corporation
    Inventors: Lance P. Encell, Mary P. Hall, Keith V. Wood, Monika G. Wood
  • Publication number: 20220064696
    Abstract: Provided herein are systems, methods, and compounds for identifying RAS binding compounds using a RAS binding agent, which comprises a RAS binding moiety and a functional element. In some embodiments, the RAS binding agent binds to one site on the RAS protein (e.g., KRAS, HRAS, or NRAS), and can be used to detect RAS binding agents that bind to the same site as well as other sites.
    Type: Application
    Filed: August 27, 2021
    Publication date: March 3, 2022
    Applicant: Promega Corporation
    Inventors: Matthew B. Robers, Joel R. Walker, James Vasta, Cesear Corona
  • Patent number: 11261478
    Abstract: Provided herein are inhibitor-resistant luciferase mutants, and methods of use thereof. In particular, luciferase mutants are provided that are thermal stable and exhibit improved stability in the presence of luciferin break-down products, such as dehydroluciferin. Further provided are assay systems comprising inhibitor-resistant luciferase mutants and amino acid sequences of the inhibitor-resistant luciferase mutants.
    Type: Grant
    Filed: October 13, 2017
    Date of Patent: March 1, 2022
    Assignee: Promega Corporation
    Inventors: Ce Shi, Thomas Kirkland, Poncho Meisenheimer, Lance P. Encell, Mary Hall
  • Patent number: 11214822
    Abstract: Methods, kits and compositions containing a mixture of D-luciferin and L-luciferin for light generation with luciferase are disclosed that have improved stability when stored over time. The mixture of D-luciferin and L-luciferin can be used to detect the presence or amount of ATP or of luciferase in a sample.
    Type: Grant
    Filed: November 1, 2019
    Date of Patent: January 4, 2022
    Assignee: PROMEGA CORPORATION
    Inventors: Michael P. Valley, James J. Cali, Brock Binkowski, Christopher Todd Eggers, Keith V. Wood
  • Patent number: 11192894
    Abstract: Described are coelenterazine analogues, methods for making the analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays.
    Type: Grant
    Filed: December 23, 2019
    Date of Patent: December 7, 2021
    Assignee: PROMEGA CORPORATION
    Inventors: Mary Hall, Thomas Kirkland, Poncho Meisenheimer, Rachel Friedman Ohana, Anton Shakhmin, Joel R. Walker, Wenhui Zhou
  • Patent number: 11164144
    Abstract: A multi-tenant, RFID system that may be Cloud based or run on a local area network (LAN) for distributed RFID devices and RFID applications. The RFID system a central abstraction and translation layer between RFID devices installed in geographically diverse locations and applications. RFID devices initiate communication to a Cloud or LAN network over to send events and receive commands. RFID applications can receive RFID tag data, device health, and requested and derived events from the RFID system to automatically run processes based on the provided data. Applications manage RFID devices and settings in the RFID system using command and configuration interfaces.
    Type: Grant
    Filed: February 1, 2019
    Date of Patent: November 2, 2021
    Assignee: Promega Corporation
    Inventors: Mark Sehmer, Travis Phillips, Mike Lohmeier
  • Patent number: 11136615
    Abstract: Disclosed herein are compositions and methods for stabilizing a benzothiazole luciferin analog such as D-luciferin and 6-amino-D-luciferin. The compositions may include the benzothiazole luciferin analog, a thionucleobase compound of formula (I), and a liquid medium, in which the thionucleobase is present in an amount effective to stabilize the luminogenic composition against decomposition. The methods provided herein may stabilize the benzothiazole luciferin analog against decomposition by contacting the benzothiazole luciferin analog with an effective amount of the thionucleobase compound in the presence of a liquid medium. Also provided herein is a kit containing the composition.
    Type: Grant
    Filed: August 2, 2018
    Date of Patent: October 5, 2021
    Assignee: PROMEGA CORPORATION
    Inventors: Ce Shi, Mary P. Hall, Thomas A. Kirkland, Lance P. Encell, Poncho Meisenheimer, Wenhui Zhou
  • Patent number: 11072811
    Abstract: The present invention provides haloalkane substrates, and linkers for connecting such substrates to functional elements (e.g., tags, labels, surfaces, etc.). Substrates and linkers described herein find use, for example, in labeling, detection, and immobilization of proteins, cells, and molecules. In particular, the linkers provided herein find use within substrates for dehalogenase variants that form covalent bonds with their haloalkane substrates.
    Type: Grant
    Filed: March 13, 2014
    Date of Patent: July 27, 2021
    Assignee: PROMEGA CORPORATION
    Inventors: Carolyn W. Hitko, Thomas Kirkland, Poncho Meisenheimer, Rachel Friedman Ohana, Harry Tetsuo Uyeda, Ji Zhu
  • Patent number: 11072812
    Abstract: The present invention provides haloalkane substrates, and linkers for connecting such substrates to functional elements (e.g., tags, labels, surfaces, etc.). Substrates and linkers described herein find use, for example, in labeling, detection, and immobilization of proteins, cells, and molecules. In particular, the linkers provided herein find use within substrates for dehalogenase variants that form covalent bonds with their haloalkane substrates.
    Type: Grant
    Filed: March 13, 2014
    Date of Patent: July 27, 2021
    Assignee: PROMEGA CORPORATION
    Inventors: Carolyn W. Hitko, Thomas Kirkland, Poncho Meisenheimer, Rachel Friedman Ohana, Harry Tetsuo Uyeda, Ji Zhu
  • Patent number: 11066692
    Abstract: Provided herein are methods for the real-time monitoring of an intracellular event or response. In particular, the methods provided herein monitor the conversion of a pro-substrate to a substrate for a protein sensor as a result of an intracellular event or response.
    Type: Grant
    Filed: September 21, 2018
    Date of Patent: July 20, 2021
    Assignee: Promega Corporation
    Inventors: James J. Cali, Sarah Duellman, Jolanta Vidugiriene, Wenhui Zhou
  • Patent number: 11028424
    Abstract: A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.
    Type: Grant
    Filed: March 20, 2019
    Date of Patent: June 8, 2021
    Assignee: Promega Corporation
    Inventors: Keith V. Wood, Georgyi V. Los, Robert F. Bulleit, Dieter Klaubert, Mark McDougall, Chad Zimprich
  • Patent number: 11015216
    Abstract: Described are substituted imidazo[1,2-a]pyrazine compounds, which are coelenterazine analogues, kits comprising the compounds, and methods of using the compounds for the detection of luminescence in luciferase-based assays. Also described are methods of making the compounds, such as a method using aminopyrazine acetophosphonates as synthesis intermediates.
    Type: Grant
    Filed: May 4, 2020
    Date of Patent: May 25, 2021
    Assignee: PROMEGA CORPORATION
    Inventors: Anton Shakhmin, Thomas Kirkland, Joel Walker, Thomas Machleidt, Mary Hall, Keith V. Wood
  • Patent number: 11010569
    Abstract: A system includes a processor, a barcode reader, and an RFID R/W component. The barcode reader scans a barcode of an item to generate barcode information, and communicates the barcode information to the processor. The RFID R/W component receives, from the processor, a serialization number corresponding to the barcode information. The RFID R/W component then writes the serialization number to an RFID tag of the item.
    Type: Grant
    Filed: August 5, 2019
    Date of Patent: May 18, 2021
    Assignee: PROMEGA CORPORATION
    Inventors: Matt Tourdot, Brian George, Dillon Biermeier, John Kuehl
  • Patent number: 10976312
    Abstract: The present invention provides compositions and methods for capture and identification of the cellular targets of a bioactive agent. In particular, provided herein are bioactive agents tethered to capture ligand, cellular targets (optionally tagged with a reporter), capture proteins (optionally present as capture fusions), surfaces (e.g., displaying, capture ligands, capture proteins, or capture fusions), and methods of capturing and identifying the cellular targets of a bioactive agent therewith.
    Type: Grant
    Filed: January 10, 2017
    Date of Patent: April 13, 2021
    Assignee: Promega Corporation
    Inventors: Carolyn W. Hitko, Robin Hurst, Thomas Kirkland, Dieter Klaubert, Poncho Meisenheimer, Nidhi Nath, Rachel Friedman Ohana, Paul Otto, Marjeta Urh, Harry Tetsuo Uyeda, Keith Wood, Ji Zhu
  • Patent number: 10928400
    Abstract: Provided herein are systems and methods for characterizing target/ligand engagement. In particular, luciferase-labeled polypeptide targets are used to detect or quantify target/ligand engagement (e.g., within a cell or cell lysate).
    Type: Grant
    Filed: February 11, 2020
    Date of Patent: February 23, 2021
    Assignee: Promega Corporation
    Inventors: Melanie Dart, Lance P. Encell, Thomas Kirkland, Thomas Machleidt, Matthew Robers, Brock F. Binkowski, Keith Wood, Ce Shi
  • Patent number: 10913972
    Abstract: Described are luciferin analogues, methods for making the analogues, kits including the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays.
    Type: Grant
    Filed: July 19, 2019
    Date of Patent: February 9, 2021
    Assignee: Promega Corporation
    Inventors: Lance P. Encell, Mary P. Hall, Michael Killoran, Thomas A. Kirkland, Poncho Meisenheimer, Ce Shi
  • Patent number: 10908135
    Abstract: The present invention provides reagents for instrumentation quality control and methods of use thereof. In particular, sets of peptides or other molecules are provided for evaluating the performance of instruments with mass spectrometry (MS) and/or liquid chromatography (LC) functionalities.
    Type: Grant
    Filed: August 23, 2019
    Date of Patent: February 2, 2021
    Assignee: Promega Corporation
    Inventors: Joshua Jacques Coon, Michael M. Rosenblatt, Marjeta Urh
  • Patent number: 10900939
    Abstract: The present invention provides reagents for instrumentation quality control and methods of use thereof. In particular, sets of peptides or other molecules are provided for evaluating the performance of instruments with mass spectrometry (MS) and/or liquid chromatography (LC) functionalities.
    Type: Grant
    Filed: February 14, 2019
    Date of Patent: January 26, 2021
    Assignee: Promega Corporation
    Inventors: Joshua Jacques Coon, Michael M. Rosenblatt, Marjeta Urh
  • Patent number: 10889849
    Abstract: The present invention provides an assay for detection of oxidized glutathione (GSSG).
    Type: Grant
    Filed: October 5, 2017
    Date of Patent: January 12, 2021
    Assignee: PROMEGA CORPORATION
    Inventors: Fen Huang, Dieter Klaubert, John Shultz, Wenhui Zhou
  • Patent number: 10882035
    Abstract: Provided herein are palladium (Pd) catalysts with improved performance in biological environments. In particular, formulations, methods of preparations, and storage conditions are provided that provide improved performance of Pd catalysts under protein-rich conditions.
    Type: Grant
    Filed: May 10, 2017
    Date of Patent: January 5, 2021
    Assignee: Promega Corporation
    Inventors: Sergiy Levin, Rachel Friedman Ohana, Thomas Kirkland, Keith Wood