Patents Assigned to ProteinSimple
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Patent number: 11953436Abstract: Embodiments described herein relate to devices, and methods for quantifying thiol content in a sample containing a mixture of proteins or protein isoforms. The method includes conjugating a portion of the sample with free thiol detection binders, separating the contents in the portion of the sample into separated protein isoforms, detecting fluorescence signals associated with each separated protein isoform, and quantifying, based on the fluorescence signals, a relative amount of free thiol associated with each separated protein isoform. In some instances, the method includes quantifying the amount of each separated protein isoform based on absorbance signals associated with each separated protein isoform. In some instances, the fluorescence and/or absorbance signals associated with protein isoforms conjugated with detection binders can be compared with the corresponding signals associated with unconjugated protein isoforms.Type: GrantFiled: February 22, 2021Date of Patent: April 9, 2024Assignee: ProteinSimpleInventors: James McElroy, Christopher Heger
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Patent number: 11933759Abstract: An apparatus includes a body portion that defines a reservoir and a set of substantially flexible capillaries. The set of substantially flexible capillaries are fixedly coupled to the body portion and in fluid communication with the reservoir. A connector is configured to be coupled to the body portion to be in fluid communication with the reservoir and the set of substantially flexible capillaries. The connector is further configured to be coupled to a vacuum source. The apparatus is arranged such that at least a part of the body portion is electrically conductive. Methods for separating and detecting an analyte from a biological sample with the apparatus are also provided. For example, methods for separating and detecting one or more proteins from a cellular lysate or a purified protein are also provided.Type: GrantFiled: September 18, 2017Date of Patent: March 19, 2024Assignee: ProteinSimpleInventors: Tom W. Yang, David John Roach
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Patent number: 11913904Abstract: Embodiments described herein generally relate to cartridges suitable for performing electrophoretic separation of analytes. Cartridges described herein are particularly well suited for reuse. Cartridges described herein can include a reservoir disposed between a capillary and a container containing a run buffer. The reservoir can inhibit run buffer from intruding into the capillary, thereby allowing repeated electrophoretic separations to be more consistent, more accurate, and/or more reliable.Type: GrantFiled: July 26, 2021Date of Patent: February 27, 2024Assignee: ProteinSimpleInventors: Hui Xu, Uyen Nguyen
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Publication number: 20240052435Abstract: Embodiments disclosed include systems, devices, and methods for analysis of samples containing particles used for gene delivery to determine a quality of the sample and/or an indication that the gene delivery particles are in a full, partial, and/or empty state. The present disclosure also relates to determining a protein and/or NA content in samples with known proportions of gene delivery particles in a full, partial, and/or empty state and based on the determination, establish a relationship between NA content and proportions of gene delivery particles in a full state. The present disclosure also relates to using such an established relationship to predict a proportion of the gene delivery particles in a full, partial, and/or empty state in test samples having the gene delivery particles in an unknown state.Type: ApplicationFiled: October 24, 2023Publication date: February 15, 2024Applicant: ProteinSimpleInventors: Jiaqi WU, Christopher HEGER
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Patent number: 11827946Abstract: Embodiments disclosed include systems, devices, and methods for analysis of samples containing particles used for gene delivery to determine a quality of the sample and/or an indication that the gene delivery particles are in a full, partial, and/or empty state. The present disclosure also relates to determining a protein and/or NA content in samples with known proportions of gene delivery particles in a full, partial, and/or empty state and based on the determination, establish a relationship between NA content and proportions of gene delivery particles in a full state. The present disclosure also relates to using such an established relationship to predict a proportion of the gene delivery particles in a full, partial, and/or empty state in test samples having the gene delivery particles in an unknown state.Type: GrantFiled: December 22, 2022Date of Patent: November 28, 2023Assignee: ProteinSimpleInventors: Jiaqi Wu, Christopher Heger
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Publication number: 20230272003Abstract: Some embodiments described herein relate to a method that includes separating an analyte-containing sample via electrophoresis in a capillary. The capillary is loaded with a chemiluminescence agent, such as luminol, that is configured to react with the analyte (e.g., HRP-conjugated proteins) to produce a signal indicative of a concentration and/or quantity of analyte at each location along the length of the capillary. A first image of the capillary containing the analytes and the chemiluminescence agent is captured over a first period of time. A second image of the capillary containing the analytes and the chemiluminescence agent is captured over a second, longer, period of time. A concentration and/or quantity of a first population of analytes at a first location is determined using the first image, and a concentration and/or quantity of a second population of analytes at a second location is determined using the second image.Type: ApplicationFiled: April 17, 2023Publication date: August 31, 2023Applicant: ProteinSimpleInventors: Hui XU, Kenneth SWARTZ
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Patent number: 11726058Abstract: A system includes a housing, a cartridge retainer disposed within the housing, a detection assembly disposed within the housing, and a reagent tray holder movably disposed in the housing. The cartridge retainer configured to receive a capillary cartridge having a capillary. The detection assembly includes at least one emitter, a first detector, and a second detector. The detection assembly is configured to transition between a first configuration, in which the first detector detects a first output of the at least one emitter, and a second configuration, in which the second detector detects a second output of the at least one emitter. The reagent tray holder is configured to move relative to the cartridge retainer to place the capillary of the capillary cartridge in fluid communication with a reagent volume.Type: GrantFiled: October 5, 2020Date of Patent: August 15, 2023Assignee: ProteinSimpleInventors: David J. Roach, Tom Weisan Yang, Hui Xu
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Patent number: 11649263Abstract: Some embodiments described herein relate to a method that includes separating an analyte-containing sample via electrophoresis in a capillary. The capillary is loaded with a chemiluminescence agent, such as luminol, that is configured to react with the analyte (e.g., HRP-conjugated proteins) to produce a signal indicative of a concentration and/or quantity of analyte at each location along the length of the capillary. A first image of the capillary containing the analytes and the chemiluminescence agent is captured over a first period of time. A second image of the capillary containing the analytes and the chemiluminescence agent is captured over a second, longer, period of time. A concentration and/or quantity of a first population of analytes at a first location is determined using the first image, and a concentration and/or quantity of a second population of analytes at a second location is determined using the second image.Type: GrantFiled: May 28, 2019Date of Patent: May 16, 2023Assignee: ProteinSimpleInventors: Hui Xu, Kenneth Swartz
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Publication number: 20230126263Abstract: Embodiments disclosed include systems, devices, and methods for analysis of samples containing particles used for gene delivery to determine a quality of the sample and/or an indication that the gene delivery particles are in a full, partial, and/or empty state. The present disclosure also relates to determining a protein and/or NA content in samples with known proportions of gene delivery particles in a full, partial, and/or empty state and based on the determination, establish a relationship between NA content and proportions of gene delivery particles in a full state. The present disclosure also relates to using such an established relationship to predict a proportion of the gene delivery particles in a full, partial, and/or empty state in test samples having the gene delivery particles in an unknown state.Type: ApplicationFiled: December 22, 2022Publication date: April 27, 2023Applicant: ProteinSimpleInventors: Jiaqi WU, Christopher HEGER
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Publication number: 20230075533Abstract: Embodiments disclosed include systems, devices, and methods for analysis of samples containing particles used for gene delivery to determine a quality of the sample and/or an indication that the gene delivery particles are in a full, partial, and/or empty state. The present disclosure also relates to determining a protein and/or NA content in samples with known proportions of gene delivery particles in a full, partial, and/or empty state and based on the determination, establish a relationship between NA content and proportions of gene delivery particles in a full state. The present disclosure also relates to using such an established relationship to predict a proportion of the gene delivery particles in a full, partial, and/or empty state in test samples having the gene delivery particles in an unknown state.Type: ApplicationFiled: September 3, 2021Publication date: March 9, 2023Applicant: ProteinSimpleInventors: Jiaqi WU, Christopher HEGER
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Publication number: 20230062228Abstract: Embodiments include systems, apparatuses, and methods to efficiently separate analytes in a sample and elute fractions of the separated analytes. In some embodiments, a method includes introducing a sample in a capillary with a first end ionically coupled to a first running buffer and a second end ionically coupled to a second running buffer to form a pH gradient. The method includes applying a voltage between the first running buffer and the second running buffer, to separate a plurality of analytes in the sample. The method includes disposing the second end of the capillary in a collection well including a chemical mobilizer and applying a voltage to elute one or more analytes from the plurality of analytes in the sample, that have been separated, into the collection well. Embodiments include detection methods to monitor separation of analytes, mobilization of analytes, and/or elution of fractions containing analytes.Type: ApplicationFiled: July 21, 2022Publication date: March 2, 2023Applicant: ProteinSimpleInventors: Cheng ZHOU, Hui XU, Roland Y. WANG, David J. ROACH, Jessica DERMODY, Tom Weisan YANG, Jiaqi WU
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Patent number: 11535900Abstract: Embodiments disclosed include systems, devices, and methods for analysis of samples containing particles used for gene delivery to determine a quality of the sample and/or an indication that the gene delivery particles are in a full, partial, and/or empty state. The present disclosure also relates to determining a protein and/or NA content in samples with known proportions of gene delivery particles in a full, partial, and/or empty state and based on the determination, establish a relationship between NA content and proportions of gene delivery particles in a full state. The present disclosure also relates to using such an established relationship to predict a proportion of the gene delivery particles in a full, partial, and/or empty state in test samples having the gene delivery particles in an unknown state.Type: GrantFiled: December 17, 2021Date of Patent: December 27, 2022Assignee: ProteinSimpleInventors: Jiaqi Wu, Christopher Heger
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Patent number: 11420202Abstract: Embodiments include systems, apparatuses, and methods to efficiently separate analytes in a sample and elute fractions of the separated analytes. In some embodiments, a method includes introducing a sample in a capillary with a first end ionically coupled to a first running buffer and a second end ionically coupled to a second running buffer to form a pH gradient. The method includes applying a voltage between the first running buffer and the second running buffer, to separate a plurality of analytes in the sample. The method includes disposing the second end of the capillary in a collection well including a chemical mobilizer and applying a voltage to elute one or more analytes from the plurality of analytes in the sample, that have been separated, into the collection well. Embodiments include detection methods to monitor separation of analytes, mobilization of analytes, and/or elution of fractions containing analytes.Type: GrantFiled: August 31, 2021Date of Patent: August 23, 2022Assignee: ProteinSimpleInventors: Cheng Zhou, Hui Xu, Roland Y. Wang, David J. Roach, Jessica Dermody, Tom Weisan Yang, Jiaqi Wu
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Publication number: 20220260525Abstract: A system for assaying a biological sample for a presence of a target analyte includes an assaying device and a computer controller. The assaying device includes a housing, a receptacle disposed in the housing, and a source of activation energy. The receptacle is configured to accept an electrophoresis cell. The electrophoresis cell has a recess area configured to accept a chip configured to accept the biological sample. The chip includes a polymeric separation medium with activatable functional groups that covalently bond to the target analyte when activated. The source of activation energy is configured to supply activation energy to activate the activatable functional groups. The computer controller is operably coupled to the source of activation energy and is configured to activate the source of activation energy to direct an application of activation energy to the polymeric separation medium to activate the activatable functional groups.Type: ApplicationFiled: December 23, 2021Publication date: August 18, 2022Applicant: ProteinSimpleInventors: Joshua I. MOLHO, Kelly GARDNER, Danh C. TRAN, Eric JABART, David NILSON, Yuri KOUCHNIR, Michael HUSTON, Colin B. KENNEDY, David John ROACH
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Publication number: 20220236221Abstract: Embodiments described herein relate to single-channel free-flow electrophoresis devices or apparatuses, and methods for separating and collecting analytes of interest from a sample by sequentially adjusting the pH value of the electrolyte buffers and separating the analyte of interest according to the corresponding isoelectric points of the analyte of interest. The method includes flowing a sample through a single center channel, applying an electric field perpendicular to a flow direction of the sample via an anolyte channel and a catholyte channel that are parallel to the center channel, and then collecting a fraction of the analyte of interest in accordance with their respective isoelectric points.Type: ApplicationFiled: April 11, 2022Publication date: July 28, 2022Applicant: ProteinSimpleInventors: David John ROACH, Hui XU, Tom Weisan YANG
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Publication number: 20220155284Abstract: Some embodiments described herein relate to systems and methods operable to combine immunoassay and Total Protein techniques in a single sample run. Some embodiments described herein allow for multiple sequential immunoassays to be performed in the same microfluidic device. Some embodiments described herein relate to stripping reagents operable to remove primary antibodies associated with immunoassays. Such stripping reagents can allow for additional immunoassays and/or Total Protein assays to be performed on the same sample.Type: ApplicationFiled: January 31, 2022Publication date: May 19, 2022Applicant: ProteinSimpleInventors: Irina Georgievna KAZAKOVA, Crystal TRAN, Jessica DERMODY, Annegret BOGE, Tom Weisan YANG
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Patent number: 11237131Abstract: A system for assaying a biological sample for a presence of a target analyte includes an assaying device and a computer controller. The assaying device includes a housing, a receptacle disposed in the housing, and a source of activation energy. The receptacle is configured to accept an electrophoresis cell. The electrophoresis cell has a recess area configured to accept a chip configured to accept the biological sample. The chip includes a polymeric separation medium with activatable functional groups that covalently bond to the target analyte when activated. The source of activation energy is configured to supply activation energy to activate the activatable functional groups. The computer controller is operably coupled to the source of activation energy and is configured to activate the source of activation energy to direct an application of activation energy to the polymeric separation medium to activate the activatable functional groups.Type: GrantFiled: May 20, 2016Date of Patent: February 1, 2022Assignee: ProteinSimpleInventors: Joshua I. Molho, Kelly Gardner, Danh C. Tran, Eric Jabart, David Nilson, Yuri Kouchnir, Michael Huston, Colin B. Kennedy, David John Roach
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Patent number: 11237164Abstract: Some embodiments described herein relate to systems and methods operable to combine immunoassay and Total Protein techniques in a single sample run. Some embodiments described herein allow for multiple sequential immunoassays to be performed in the same microfluidic device. Some embodiments described herein relate to stripping reagents operable to remove primary antibodies associated with immunoassays. Such stripping reagents can allow for additional immunoassays and/or Total Protein assays to be performed on the same sample.Type: GrantFiled: July 17, 2020Date of Patent: February 1, 2022Assignee: ProteinSimpleInventors: Irina Georgievna Kazakova, Crystal Tran, Jessica Dermody, Annegret Boge, Tom Weisan Yang
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Publication number: 20210356427Abstract: Embodiments described herein generally relate to cartridges suitable for performing electrophoretic separation of analytes. Cartridges described herein are particularly well suited for reuse. Cartridges described herein can include a reservoir disposed between a capillary and a container containing a run buffer. The reservoir can inhibit run buffer from intruding into the capillary, thereby allowing repeated electrophoretic separations to be more consistent, more accurate, and/or more reliable.Type: ApplicationFiled: July 26, 2021Publication date: November 18, 2021Applicant: ProteinSimpleInventors: Hui XU, Uyen NGUYEN
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Patent number: 11143650Abstract: Some embodiments described herein relate to systems and methods operable to combine immunoassay and Total Protein techniques in a single sample run. Some embodiments described herein allow for multiple sequential immunoassays to be performed in the same microfluidic device. Some embodiments described herein relate to stripping reagents operable to remove primary antibodies associated with immunoassays. Such stripping reagents can allow for additional immunoassays and/or Total Protein assays to be performed on the same sample.Type: GrantFiled: July 17, 2020Date of Patent: October 12, 2021Assignee: ProteinSimpleInventors: Irina Georgievna Kazakova, Crystal Tran, Jessica Dermody, Annegret Boge, Tom Yang