Abstract: Methods of transferring bio-molecular components of individual cells in a biological sample to a solid porous substate, as well as methods of examining or detecting one or more bio-molecular components of individual cells in a biological sample. The method transferring including contacting the biological sample to a first side of the porous solid substrate having a plurality of interstices or pores extending contiguously from the first side to a second side, transferring and affixing the bio-molecular components of the biological sample to the interstices or pores of the solid substrate, and the method of examining or detecting includes detecting one or more of the bio-molecular components of the biological sample transferred to the solid porous substrate.