Abstract: The present invention relates to methods for determining a binding and/or functional interaction of a protein of interest with a nucleosomal substrate wherein at least one of the histone types of the nucleosomal substrate has a homogenous post-translational modification pattern. Further, the invention relates to nucleosomal substrates, wherein at least one of the histone types of the nucleosomal substrate has a homogenous post-translational modification pattern, and to methods for providing such nucleosomal substrates.

Abstract: An apparatus for generating a defined environment for particle-shaped samples, comprises a support element with a rest end for a particle-shaped sample and an apparatus for generating a humid gas flow at a mouth end thereof. The mouth end is directed to the rest end. A gas provider provides gas having a first dew-point temperature. A cooler cools the gas to a cooler temperature under condensation of moisture to adjust a second dew-point temperature of the gas. A guide guides the gas with the second dew-point temperature to the mouth end, preventing condensation of moisture from the gas. A controller adjusts the relative humidity of the gas at the mouth end by adjusting the cooler temperature and adjusting the temperature of the gas at the mouth end.

Abstract: The present invention relates to a method for treating a crystal with a solution, wherein the solution contains one or more molecule species and wherein the molecules of the molecule species typically have a molecular weight of <500 Da. By means of this method, small molecules or molecule fragments weakly binding on target structures can be identified and their binding position can be determined by means of subsequent X-ray crystallographic examinations.