Abstract: An optical scanning microscope includes an illumination system (160) and an objective lens (134) operable together to provide the excitation radiation (152), (154) in a focal volume (124) at sufficient intensity to cause non-linear emission of emission radiation from a sample in the focal volume. The objective is an aspheric objective configured to focus the excitation radiation without, or with minimal, spherical aberration. The objective lens (134) is scanned by an objective scanner (130), (132). In this example an x-y transducer (xyXD) (130) is connected to a kinematic flexure mechanism (132) which acts as a scanning lens mount. The objective scanner (130), (132) is operable to scan the objective (134) in two dimensions transverse with respect to the objective's optical axis so as to scan the emitting focal volume (124) in corresponding dimensions.

Abstract: An optical scanning microscope includes an illumination system (160) and an objective lens (134) operable together to provide the excitation radiation (152, 154) in a focal volume (124) at sufficient intensity to cause emission of emission radiation from a sample in the focal volume. The objective lens (134) is scanned by an objective scanner (130, 132). In this example an x-y transducer (xyXD) (130) is connected to a kinematic flexure mechanism (132) which acts as a scanning lens mount. The kinematic flexure mechanism is operable to scan the objective in two dimensions transverse with respect to the objective's optical axis so as to scan the emitting focal volume in corresponding dimensions. The kinematic flexure mechanism may be a unitary 3D-printed member.